Peptide with putative role in cytoskeletal protection

• US 7,659,366 B2
• Filed: 12/12/2006
• Issued: 02/09/2010
• Est. Priority Date: 12/12/2005
• Status: Expired due to Fees

• Current Assignee: Mohsen Amin, Richard P. Ellen
• Original Assignee: Mohsen Amin, Richard P. Ellen
• Inventors: Ellen, Richard P., Amin, Mohsen
• Primary Examiner(s): Lukton; David
• Application Number:
  US11/609,896
• Publication Number:
  US 20070167366A1
• Time in Patent Office: 1,155 Days
• Field of Search: 514/2, 530/324, 530/328
• US Class Current: 530/328
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C07K 7/06 having 5 to 11 amino acids

Abstract

The invention relates to a novel peptide conjugate useful for stabilizing the actin cytoskeleton of mammalian cells. More particularly, the invention relates to a conjugate of a peptide found within the sequence of a bacterium'"'"'s major outer sheath protein and bovine serum albumin (BSA), and the use of this conjugate for stabilizing the actin cytoskeleton of mammalian cells, and for preventing or controlling the migration of mammalian cells.

Claims

1. A peptide conjugate consisting of a carrier and one or more identical peptides, wherein each of said one or more identical peptides consists of the peptide sequence YAGKDKNNKA (SEQ ID NO:

2) and is conjugated to said carrier through the N-terminus only of said one or more identical peptides.

2. The peptide conjugate as claimed in claim 1, wherein the carrier is bovine serum albumin.

3. The peptide conjugate as claimed in claim 2, wherein said conjugate comprises an average of 6 identical peptides.

4. A method for stabilizing the actin cytoskeleton of mammalian cells comprising the step of exposing said cells to the peptide conjugate as claimed in claim 1.

5. The method as claimed in claim 4, wherein said mammalian cells are fibroblast cells.

6. A method for inhibiting migration of mammalian cells, comprising the step of exposing said mammalian cells to the peptide conjugate as claimed in claim 1.

7. The method as claimed in claim 6, wherein said mammalian cells are fibroblast cells.

8. The method as claimed in claim 6, wherein said mammalian cells are neutrophils.

9. The method as claimed in claim 6, wherein the peptide conjugate is present in a concentration of about 50 μ

g/ml.