Apparatus for aspirating and dispensing liquids in an automated analyzer

• US 7,661,326 B2
• Filed: 10/26/2006
• Issued: 02/16/2010
• Est. Priority Date: 10/26/2006
• Status: Active Grant

• Current Assignee: Beckman Coulter Incorporated (Danaher Corporation)
• Original Assignee: Beckman Coulter Incorporated (Danaher Corporation)
• Inventors: Casey, William J., Gonzalez, Carlos R., Galvez, Santiago, Li, William W., Veiner, Craig R., Del Valle, Roberto, Cano, Jose M.
• Primary Examiner(s): Raevis; Robert R
• Application Number:
  US11/553,107
• Publication Number:
  US 20080098828A1
• Time in Patent Office: 1,209 Days
• Field of Search: None
• US Class Current: 73/863.73
• CPC Class Codes: G01N 35/1097 characterised by the valves valves in general F16K

Abstract

Apparatus for aspirating and dispensing liquid samples in an analytical instrument, e.g., a hematology instrument, includes a liquid-sampling valve that, while operating to segment and position for dispensing one or more precise volumes of a liquid sample that has been aspirated into the valve by a pump, simultaneously enables the apparatus to be operated in an aspirate/dispense (suck-and-spit) mode in which a liquid sample can be selectively driven through the valve in opposite directions by a pump, e.g., a syringe pump.

Claims

1. Apparatus for aspirating and dispensing a liquid sample in an automated analytical instrument, said apparatus comprising:

(a) an aspiration probe, operably connected to a pump, and adapted to enter a sample container to aspirate a liquid sample within such container;

(b) a transport system for selectively advancing the aspiration probe in mutually perpendicular planes so that said aspiration probe can aspirate or dispense liquid from or to different containers spaced apart; and

(c) a liquid-sampling valve, operatively connected between said aspiration probe and pump, and comprising structure defining at least one aliquoting chamber for temporarily storing a precise volume of liquid sample to be dispensed;

said pump being selectively operable to either (i) draw a liquid sample through said aspiration probe and through the liquid-sampling valve to fill said aliquoting chamber, or (ii) to dispense an already aspirated liquid sample through said liquid-sampling valve and through aspiration probe, said liquid-sampling valve being selectively operable in either (i) a loading configuration in which a liquid sample aspirated by the aspiration probe under the influence of the pump will pass through the liquid-sampling valve while simultaneously filling said aliquoting chamber thereof, or (ii) a segmenting/dispensing configuration in which liquid within said aliquoting chamber is positioned to be dispensed from said liquid-sampling valve by an external force, while a liquid sample can be either aspirated or dispensed through said aspiration probe and through said liquid-sampling valve by said pump.

2. Apparatus as defined by claim 1 wherein said pump is a stepper-motor controlled syringe pump.

3. Apparatus as defined by claim 1 wherein said shear valve assembly comprises a plurality of confronting and contiguous valve pads, each of said pads having structure that cooperates with structure of an adjacent pad to define different liquid flow paths within said shear valve assembly, at least one of said pads being movable with respect to the other of said pads to define either (a) a loading configuration in which a first aliquoting chamber positioned within a first flow path and becomes filled with a liquid aspirated through said aspiration probe and through said valve assembly by said pump, and (b) a segmenting/dispensing configuration in which (i) liquid within said first aliquoting chamber is positioned to be acted upon by an external force to dispense such liquid from said first aliquoting chamber along a second flow path, and (ii) liquid can be either aspirated or dispensed through said valve assembly along a third flow path by said pump.

4. The liquid-sampling valve as defined by claim 3 wherein said first aliquoting chamber is defined by a bore hole passing through one of said valve pads.

5. The liquid-sampling valve as defined by claim 4 wherein said shear valve assembly comprises three valve pads, and wherein said bore hole is formed in a middle pad of said three valve pads.

6. The liquid-sampling valve as defined by claim 3 wherein a second aliquoting chamber is positioned in said first flow path and becomes filled with liquid aspirated through said liquid-sampling valve when said liquid-aspirating valve is its loading configuration, and wherein liquid within said second aliquoting chamber is positioned to be acted upon by an external force to dispense such liquid from said second aliquoting chamber along a fourth flow path when said liquid-sampling valve is its segmenting/dispensing configuration.