Chemical sensor device

• US 7,662,612 B2
• Filed: 05/10/2001
• Issued: 02/16/2010
• Est. Priority Date: 05/11/2000
• Status: Expired due to Fees

• Current Assignee: Panasonic Ecology Systems Company Limited (Panasonic Holdings Corporation)
• Original Assignee: Panasonic Corporation (Panasonic Holdings Corporation)
• Inventors: Nishiguchi, Masashi, Onouchi, Toru, Niwa, Kazuhiro
• Primary Examiner(s): Lam; Ann Y
• Application Number:
  US10/275,783
• Publication Number:
  US 20030180184A1
• Time in Patent Office: 3,204 Days
• Field of Search: 204/164, 204/193, 2044031-4036, 204/601, 356/244, 356300- 3, 356/311, 356/319, 356/326, 422/100, 422 50- 73, 435 4- 795, 435/800, 435804-810, 435/290, 435/283.1, 4352852-2891, 435/973, 435/290.1, 436514-547, 436/73, 436/74, 436/800, 436/149
• US Class Current: 435/287.2
• CPC Class Codes: G01N 33/5005 involving human or animal cells immunoassay G01N33/56966; immunoassays of protozoa G01N33/56905; protozoa in screening assays C12Q1/025
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 33/54393 Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding

Abstract

A sensor device is provided for performing rapid and high-sensitivity detection. The sensor device comprising at least one support for immobilizing a subject to be detected and a cell for containing a solution in which a reaction product generated from the subject to be detected is diffused. At least one reaction region having a constant concentration of the reaction product is formed by diffusion of the reaction product into the solution, and the reaction region is formed in such a manner that the reaction product is specifically detected in a predetermined measurement time.

Claims

1. A sensor device, comprising:

a cell for containing a solution in which a redox reaction product is generated;

at least one support means within the cell, each of said at least one support means including a fixing means to immobilize only one subject to be detected; and

a detection means separate from the support means for detecting a current generated from said redox reaction product, wherein;

each reaction product is generated from each of the at least one subject to be detected and the at least one subject is detected by generation of said each reaction product in a corresponding reaction region;

one or more reaction regions are formed, each reaction region having a constant concentration of each corresponding reaction product, and each reaction region being formed by diffusion of each reaction product through the solution;

the at least one subject to be detected is immobilized in at least one region, each region having a radius of 50 μ

m to 100 μ

m;

each reaction region and each corresponding region in which each subject is immobilized have substantially the same size, and each reaction region and a corresponding detection region are arranged in a relative positional relationship, such that said each reaction region and said each corresponding detection region overlap substantially significantly;

the detection means comprises one or more electrodes, each electrode corresponding to each of the one or more reaction regions in a one-to-one relationship, and each electrode corresponding to each fixing means in a one-to-one relationship; and

for the case of more than one electrode and corresponding more than one reaction region, the more than one reaction regions are separately formed by different reaction products.

2. A sensor device according to claim 1, wherein the detection means generates the detection region, and the detection region includes the reaction region.

3. A sensor device according to claim 1, wherein the detection means generates the detection region, and the detection region overlaps the reaction region.

4. A sensor device according to claim 1, wherein the detection means generates the detection region, and the detection region is included in the reaction region.

5. A sensor device according to claim 1, wherein the support is a defined region on a base plate.

6. A sensor device according to claim 1, wherein the support is a particulate.

7. A sensor device according to claim 1, wherein the support is a rod-shaped member.

8. A sensor device according to claim 1, wherein each electrode generates an electrical signal corresponding to the amount of each reaction product.

9. A sensor device according to claim 1, wherein the subject to be detected is an enzyme, and the reaction product is an enzyme reaction product.

10. A sensor device according to claim 1, wherein the subject to be detected is an antibody or an enzyme linked to a peptide.

11. A sensor device according to claim 10, wherein the subject to be detected is immobilized by an antigen.

12. A sensor device according to claim 10, wherein the subject to be detected is immobilized by an antigen-antibody complex.

13. A sensor device according to claim 1, wherein the subject to be detected is an enzyme linked to a first antibody linked to an antigen, and is immobilized by a second antibody.

14. A sensor device according to claim 1, wherein each of the one or more electrodes has a diameter of 1 mm or less.

15. A sensor device according to claim 1, wherein each of the one or more electrodes surrounds each of the at least one region on which each of the at least one subject to be detected is immobilized.

16. A sensor device according to claim 1, wherein the support is a material selected from the group consisting of glass, ceramics, noble metals, and resins.

17. A sensor device according to claim 1, further comprising means for promoting the immobilizing of the subject to be detected.

18. A sensor device according to claim 17, wherein the promoting means is a means for stirring the solution in the cell.

19. A sensor device according to claim 17, wherein the promoting means is a means for exchanging the solution in the cell.

20. A sensor device according to claim 17, wherein the promoting means is a means for supplying the solution into the cell.

21. A sensor device according to claim 17, wherein the promoting means is a means for allowing the solution to flow through the cell.