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Absolute intensity determination for a fluorescent light source in low level light imaging systems

  • US 7,663,664 B2
  • Filed: 09/18/2006
  • Issued: 02/16/2010
  • Est. Priority Date: 06/20/2001
  • Status: Active Grant
First Claim
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1. A method for generating absolute fluorescent light intensity data for a fluorescent light source being imaged at low intensity light levels, the method comprising:

  • determining a compensation factor for converting data output by a camera into absolute fluorescent intensity light data, the compensation factor corresponding to a set of imaging conditions;

    storing the compensation factor;

    calling the compensation factor based on the set of imaging conditions when the set of imaging conditions are used in imaging the fluorescent light source; and

    modifying image data, output by the camera, by the compensation factor to obtain the absolute fluorescent light intensity data for the fluorescent light source, wherein the fluorescent light source emits light with an intensity in the range of about 103 to about 1010 photons/second/centimeter squared/steradian, and wherein the fluorescent light source includes fluorescing molecules.

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