Articles of manufacture for detection of herpes simplex virus

• US 7,667,025 B2
• Filed: 05/27/2005
• Issued: 02/23/2010
• Est. Priority Date: 01/31/2001
• Status: Expired due to Term

• Current Assignee: Mayo Foundation For Medical Education And Research (Mayo Clinic)
• Original Assignee: Mayo Foundation For Medical Education And Research (Mayo Clinic)
• Inventors: Wold, Arlo, Smith, Thomas F., Uhl, Jim, Espy, Mark J.
• Primary Examiner(s): Horlick; Kenneth R.
• Application Number:
  US11/140,640
• Publication Number:
  US 20060019242A1
• Time in Patent Office: 1,733 Days
• Field of Search: 435/5, 536/24.3, 536/24.32, 536/24.33
• US Class Current: 536/24.3
• CPC Class Codes: C12Q 1/6818 involving interaction of two or more labels, e.g. resonant energy transfer
• CPC Class Codes: C12Q 1/705 for herpetoviridae, e.g. herpes simplex, varicella zoster

Abstract

The invention provides methods to detect herpes simplex virus (HSV) in biological samples and further to distinguish between HSV-1 and HSV-2. Primers and probes for the differential detection of HSV-1 and HSV-2 are provided by the invention. Articles of manufacture containing such primers and probes for detecting HSV are further provided by the invention.

Claims

1. An article of manufacture, comprising:

a pair of HSV DNA polymerase primers, wherein said pair of HSV DNA polymerase primers comprises a first HSV DNA polymerase primer and a second HSV DNA polymerase primer, wherein said first HSV DNA polymerase primer consists of the sequence 5′

-GCT CGA GTG CGA AAA AAC GTT C-3′

(SEQ ID NO;

1), and wherein said second HSV DNA polymerase primer consists of the sequence 5′

-CGG GGC GCT CGG CTA AC-3′

(SEQ ID NO;

2);

a pair of HSV DNA polymerase probes, wherein said pair of HSV DNA polymerase probes comprise a first HSV DNA polymerase probe and a second HSV DNA polymerase probe, wherein said first HSV DNA polymerase probe consists of the sequence 5′

-GCG CAC CAG ATC CAC GCC CTT GAT GAG C-3′

(SEQ ID NO;

3), and wherein said second HSV DNA polymerase probe consists of the sequence 5′

-CTT GCC CCC GCA GAT GAC GCC-3′

(SEQ ID NO;

4); and

a donor fluorescent moiety and a corresponding acceptor moiety.

3. The article of manufacture of claim 1 or claim 2, wherein said pair of HSV DNA polymerase probes are labeled with said donor fluorescent moiety and said corresponding acceptor fluorescent moiety.

4. The article of manufacture of claim 1 or claim 2, further comprising a label or package insert having instructions thereon for using said pair of HSV DNA polymerase primers and said pair of HSV DNA polymerase probes to detect the presence or absence of HSV in a biological sample.

5. The article of manufacture of claim 4, further comprising a label or package insert having instructions thereon for using said pair of HSV DNA polymerase probes to distinguish between HSV-1 and HSV-2.

2. An article of manufacture, comprising:

a pair of HSV DNA polymerase primers, wherein said pair of HSV DNA polymerase primers comprises a first HSV DNA polymerase primer and a second HSV DNA polymerase primer, wherein said first HSV DNA polymerase primer consists of the sequence 5′

-GCT CGA GTG CGA AAA AAC GTT C-3′

(SEQ ID NO;

1), and wherein said second HSV DNA polymerase primer consists of the sequence 5′

-CGG GGC GCT CGG CTA AC-3′

(SEQ ID NO;

2);

a pair of HSV DNA polymerase probes, wherein said pair of HSV DNA polymerase probes comprise a first HSV DNA polymerase probe and a second HSV DNA polymerase probe, wherein said first HSV DNA polymerase probe consists of the sequence 5′

-GTA CAT CGG CGT CAT CTG CGG GGG CAA G-3′

(SEQ ID NO;

5), and wherein said second HSV DNA polymerase probe consists of the sequence 5′

-T GCT CAT CAA GGG CGT GGA TCT GGT GC-3′

(SEQ ID NO;

6); and

a donor fluorescent moiety and a corresponding acceptor moiety.