Mesenchymal precursor cell
First Claim
1. A method of generating bone tissue in a subject comprising administering to the subject a population of cells enriched for STRO-1bright cells, or culture expanded colony-forming-unit-fibroblasts (CFU-Fs) derived therefrom, wherein such STRO-1bright cells are mesenchymal precursor cells capable of giving rise to CFU-Fs, so as to generate bone tissue in the subject.
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Abstract
A method of enriching mesenchymal precursor cells including the step of enriching for cells based on at least two markers. The markers may be either i) the presence of markers specific for mesenchymal precursor cells, ii) the absence of markers specific for differentiated mesenchymal cells, or iii) expression levels of markers specific for mesenchymal precursor cells. The method may include a first solid phase sorting step utilizing MACS recognizing expression of the antigen to the STRO-1 Mab, followed by a second sorting step utilizing two colour FACS to screen for the presence of high level STRO-1 antigen expression as well as the expression of VCAM-1.
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Citations
4 Claims
- 1. A method of generating bone tissue in a subject comprising administering to the subject a population of cells enriched for STRO-1bright cells, or culture expanded colony-forming-unit-fibroblasts (CFU-Fs) derived therefrom, wherein such STRO-1bright cells are mesenchymal precursor cells capable of giving rise to CFU-Fs, so as to generate bone tissue in the subject.
Specification