Membrane-based assay devices

• US 7,670,786 B2
• Filed: 10/22/2007
• Issued: 03/02/2010
• Est. Priority Date: 08/27/2002
• Status: Expired due to Term

• Current Assignee: Kimberly-Clark Worldwide Incorporated (Kimberly-Clark Corporation)
• Original Assignee: Kimberly-Clark Worldwide Incorporated (Kimberly-Clark Corporation)
• Inventors: Song, Xuedong, Kaylor, Rosann Marie Matthews
• Primary Examiner(s): NGUYEN, BAO THUY L
• Application Number:
  US11/876,018
• Publication Number:
  US 20080096288A1
• Time in Patent Office: 862 Days
• Field of Search: 435/7.1, 435/7.02, 435/7.21, 435/7.32, 435/239, 435/803, 435/805, 435/970, 436/514, 436/518, 436/531, 436/536, 436/533, 436/538, 436/806, 436/824
• US Class Current: 435/7.1
• CPC Class Codes: G01N 2021/6439 with indicators, stains, dyes, tags, labels, marks
• CPC Class Codes: G01N 21/274 Calibration, base line adjustment, drift correction
• CPC Class Codes: G01N 21/6408 with measurement of decay time, time resolved fluorescence
• CPC Class Codes: G01N 33/525 Multi-layer analytical elements
• CPC Class Codes: G01N 33/54326 Magnetic particles
• CPC Class Codes: G01N 33/558 using diffusion or migration of antigen or antibody immunochromatographic test strips G01N33/54387
• CPC Class Codes: G01N 33/582 with fluorescent label
• CPC Class Codes: Y10S 435/803 Physical recovery methods, e.g. chromatography, grinding
• CPC Class Codes: Y10S 435/805 Test papers
• CPC Class Codes: Y10S 435/97 Test strip or test slide
• CPC Class Codes: Y10S 436/80 Fluorescent dyes, e.g. rhodamine
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property
• CPC Class Codes: Y10S 436/824 Immunological separation techniques
• CPC Class Codes: Y10T 436/13 Tracers or tags

Abstract

A membrane-based assay device for detecting the presence or quantity of an analyte residing in a test sample is provided. The device utilizes a self-calibrated magnetic binding assay format (e.g., sandwich, competitive, etc.) that includes detection probes capable of generating a detection signal (e.g., fluorescent non-magnetic particles) and calibration probes capable of generating a calibration signal (e.g., fluorescent magnetic particles). The amount of the analyte within the test sample is proportional (e.g., directly or inversely) to the intensity of the detection signal calibrated by the intensity of the calibration signal. It has been discovered that the fluidics-based device of the present invention provides an accurate, inexpensive, and readily controllable method of determining the presence of an analyte in a test sample.

Claims

1. A method for detecting the presence or quantity of an analyte residing in a test sample, said method comprising:

i) providing a membrane-based device, said device comprising a porous membrane that defines a detection zone and is in fluid communication with;

detection probes that comprise non-magnetic particles conjugated with a first specific binding member and labeled with a detectable substance that is configured to emit a detection signal, the first specific binding member being capable of binding with the analyte;

calibration probes that comprise magnetic particles labeled with a detectable substance that is configured to produce a calibration signal; and

non-labeled magnetic particles conjugated with a second binding member, the second binding member also being capable of binding with the analyte;

ii) contacting said detection probes, said calibration probes, and said non-labeled magnetic particles with the test sample so that complexes are formed between said detection probes, analyte, and non-labeled magnetic particles;

iii) immobilizing said calibration probes and said complexes within said detection zone using a magnetic device;

iv) exciting said detection probes and said calibration probes within said detection zone, wherein said excitation causes said detection probes to emit said detection signal and said calibration probes to emit said calibration signal;

v) measuring the intensity of the detection signal at a first emission wavelength and the intensity of the calibration signal at a second emission wavelength; and

vi) comparing the intensity of the detection signal to the calibration signal, wherein the amount of the analyte within the test sample is proportional to the intensity of the detection signal calibrated by the intensity of the calibration signal.

2. The method of claim 1, wherein the device further comprises a blocking agent configured for binding to the magnetic particles of the calibration probes.

3. The method of claim 1, wherein the device further comprises a conjugate pad in fluid communication with the porous membrane and positioned upstream from the detection zone, the conjugate pad comprising the detection probes and calibration probes.

4. The method of claim 1, wherein the detectable substance of both the detection probes and the calibration probes includes a luminescent compound.

5. The method of claim 1, wherein the detectable substance of both the detection probes and the calibration probes includes a fluorescent compound.

6. The method of claim 1, wherein the first specific binding member, the second specific binding member, or both include an antigen, hapten, aptamer, antibody, or a combination thereof.

7. The method of claim 1, wherein the magnetic particles of the calibration probes are conjugated with a third specific binding member.

8. The method of claim 1, wherein said first emission wavelength is different than said second emission wavelength.

9. The method of claim 1, wherein said detection probes and said calibration probes are excited separately.

10. The method of claim 1, wherein the intensity of said detection signal and said calibration signal are measured separately.