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Monomeric red fluorescent proteins

  • US 7,671,185 B2
  • Filed: 05/15/2007
  • Issued: 03/02/2010
  • Est. Priority Date: 04/07/2004
  • Status: Active Grant
First Claim
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1. An isolated polynucleotide encoding a variant polypeptide of wild-type DsRed of SEQ ID NO:

  • 2, the variant polypeptide having reduced oligomerization relative to wild-type DsRed, the variant polypeptide comprising the amino acid substitutions K83M, K163H, and Y193H, the variant polypeptide further comprising at least one amino acid substitution selected from the group consisting of E26Y, K92T, V96S, T106E, T108Q, I125K, S131A, I180V, and M182K relative to SEQ ID NO;

    2.

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