Methods and compositions for blood pool identification, drug distribution quantification and drug release verification
First Claim
1. An in vivo method of monitoring the localization and distribution of a compound of interest to a desired site in an organism by magnetic resonance imaging, the method comprising:
- (a) administering an envirosensitive liposome composition to a subject, the composition comprising;
(i) a contrast agent;
(ii) a compound of interest; and
(iii) an envirosensitive liposome encapsulating the contrast agent and the compound of interest; and
(b) monitoring the localization and distribution of the compound of interest to a desired site by magnetic resonance imaging, wherein the monitoring comprises monitoring release of the contents of the liposome at the desired site by monitoring an increase in the presence of the contrast agent released from the liposome at the desired site as the contents of the liposome are being released at the desired site.
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Accused Products
Abstract
Methods and compositions useful for detecting an in vivo blood pool, monitoring the distribution of a compound of interest to a desired site in an organism by magnetic resonance imaging, monitoring the accumulation of a compound of interest at a desired site in vivo by magnetic resonance imaging, and monitoring the release of liposomal contents to an external stimulus at a desired site in vivo by magnetic resonance imaging are disclosed. Some compositions comprise envirosensitive or non-sensitive liposomes. Contrast agents, such as manganese-based compounds, are also disclosed.
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Citations
26 Claims
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1. An in vivo method of monitoring the localization and distribution of a compound of interest to a desired site in an organism by magnetic resonance imaging, the method comprising:
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(a) administering an envirosensitive liposome composition to a subject, the composition comprising; (i) a contrast agent; (ii) a compound of interest; and (iii) an envirosensitive liposome encapsulating the contrast agent and the compound of interest; and (b) monitoring the localization and distribution of the compound of interest to a desired site by magnetic resonance imaging, wherein the monitoring comprises monitoring release of the contents of the liposome at the desired site by monitoring an increase in the presence of the contrast agent released from the liposome at the desired site as the contents of the liposome are being released at the desired site. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11)
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12. A method of monitoring the accumulation of a compound of interest at a desired site in vivo by magnetic resonance imaging, the method comprising:
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(a) administering to a subject an envirosensitive liposome composition comprising; (i) a contrast agent; (ii) a compound of interest; and (iii) an envirosensitive liposome encapsulating the contrast agent and the compound of interest; and (b) monitoring the accumulation of the compound of interest at a desired site by magnetic resonance imaging, wherein the monitoring comprises monitoring release of the contents of the liposome at the desired site by monitoring an increase in the presence of the contrast acient released from the liposome at the desired site as the contents of the liposome are being released at the desired site. - View Dependent Claims (13, 14, 15, 16, 17, 18)
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19. A method of generating a heating profile of a site of interest, the method comprising:
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(a) administering to a subject a thermosensitive liposome composition comprising; (i) a contrast agent; and (ii) a thermosensitive liposome encapsulating the contrast agent and the compound of interest and having a melting temperature Tm; (b) heating a site of interest in a subject; (c) monitoring release of the contrast agent from the thermosensitive liposome using magnetic resonance imaging, wherein the monitoring comprises monitoring release of the contents of the liposome at the desired site by monitoring an increase in the presence of the contrast agent released from the liposome at the desired site as the contents of the liposome are being released at the desired site; (d) generating in real time a heating profile of the site of interest, wherein heating of an area to a temperature of at least Tm is indicated by release of contrast agent. - View Dependent Claims (20, 21, 22, 23, 24)
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25. An in vivo method of monitoring the localization and distribution of a compound of interest to a desired site in an organism by magnetic resonance imaging, the method comprising:
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(a) administering an envirosensitive liposome composition to a subject, the composition comprising; (i) a contrast agent; (ii) a compound of interest; and (iii) an envirosensitive liposome encapsulating the contrast agent and the compound of interest; and (b) monitoring in real time the localization and distribution of the compound of interest to a desired site by magnetic resonance imaging, wherein the monitoring comprises monitoring release of the contents of the liposome at the desired site by monitoring an increase in the presence of the contrast agent released from the liposome at the desired site as the contents of the liposome are being released at the desired site.
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26. A method of monitoring the accumulation of a compound of interest at a desired site in vivo by magnetic resonance imaging, the method comprising:
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(a) administering to a subject an envirosensitive liposome composition comprising; (i) a contrast agent; (ii) a compound of interest; and (iii) an envirosensitive liposome encapsulating the contrast agent and the compound of interest; and (b) monitoring in real time the accumulation of the compound of interest at a desired site by magnetic resonance imaging, wherein the monitoring comprises monitoring release of the contents of the liposome at the desired site by monitoring an increase in the presence of the contrast agent released from the liposome at the desired site as the contents of the liposome are being released at the desired site.
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Specification