Gels with predetermined conductivity used in electroporation of tissue

• US 7,674,249 B2
• Filed: 10/16/2007
• Issued: 03/09/2010
• Est. Priority Date: 10/16/2006
• Status: Active Grant

• Current Assignee: Regents of the University of California (University of California)
• Original Assignee: Regents of the University of California (University of California)
• Inventors: Rubinsky, Boris, Ivorra, Antoni
• Primary Examiner(s): Sirmons; Kevin C
• Assistant Examiner(s): Mehta; Bhisma
• Application Number:
  US11/872,985
• Publication Number:
  US 20080214986A1
• Time in Patent Office: 875 Days
• Field of Search: 604/20, 604/21, 604/500, 604/501, 604/506, 604/507, 604/508, 604/511
• US Class Current: 604/500
• CPC Class Codes: A61K 9/0009 involving or responsive to electricity, magnetism or acoustic waves; Galenical aspects of sonophoresis, iontophoresis, electroporation or electroosmosis microelectromechanical systems A61K9/0097
• CPC Class Codes: A61K 9/0024 Solid, semi-solid or solidifying implants, which are implanted or injected in body tissue compositions for intravenous administration, normal injectable solutions or dispersions for, e.g. subcutaneous administration A61K9/0019; brain implants A61K9/0085; (coated) prostheses, catheters or stents A61L
• CPC Class Codes: A61M 5/329 characterised by features of the needle shaft
• CPC Class Codes: A61N 1/042 Material of the electrode
• CPC Class Codes: A61N 1/327 for enhancing the absorption properties of tissue, e.g. by electroporation

Abstract

Gel compositions with adjusted conductivity are disclosed which compositions are used for directing reversible electroporation and irreversible electroporation of cells and tissue. The gel compositions are also used in a similar manner in order to carry out thermotherapy on cells and tissues.

Claims

1. A method of carrying out electroporation of tissue, comprising:

inserting a gel into a patient by injection wherein the gel has a predetermined conductivity relative to surrounding tissue, the gel being semi-solid, elastic material comprised of matter in a colloidal state;

applying current across tissue in an amount so that cells of the tissue are subjected to electroporation;

wherein the conductivity of the gel is sufficiently below that of the surrounding tissue such that when current is applied substantially no current flows through the gel and the current is directed to a specific targeted area of tissue to be electroporated.

2. The method of claim 1, wherein the gel is comprised of a therapeutically active drug.

3. The method of claim 1, wherein the gel is a hydrogel comprised of water and solid phase polymer chains.

4. The method of claim 1, wherein the conductivity of the gel is adjusted by incorporating a predetermined concentration of ions in the gel.

5. The method of claim 4, wherein ions are derived from NaCl in the gel.

6. The method of claim 1 wherein the electroporation is reversible.

7. A method of carrying out electroporation of tissue, comprising:

inserting a gel into a patient by injection wherein the gel has a predetermined conductivity relative to surrounding tissue the gel being semi-solid, elastic material comprised of matter in a colloidal state;

applying current across tissue in an amount so that cells of the tissue are subjected to electroporation;

wherein the conductivity of the gel is substantially the same as that of surrounding tissue and when current is applied a homogeneous electrical field is applied and a specific area of targeted tissue is electroporated.

8. A method of carrying out electroporation of tissue, comprising:

inserting a gel into tissue in a patient by injection wherein the gel has a predetermined conductivity relative to tissue surrounding the gel, wherein the gel is a semi-solid, elastic material comprised of matter in a colloidal state;

applying current across tissue in an amount so that cells of the tissue are subjected to electroporation;

wherein the conductivity of the gel is predetermined by incorporating a known concentration of ions in the gel.

9. The method of claim 8, wherein the ions are derived from NaCl dissolved in the gel and wherein the conductivity of the gel is sufficiently below that of the surrounding tissue such that when current is applied substantially no current flows through the gel and the current is directed to a specific targeted area of tissue to be electroporated.

10. The method of claim 8, wherein the gel is a hydrogel comprised of water and solid phase polymer chains and wherein the conductivity of the gel is substantially the same as that of surrounding tissue and when current is applied a homogeneous electrical field is applied and a specific area of targeted tissue is electroporated.

11. The method of claim 8, wherein the gel is comprised of a therapeutically active drug.

12. The method of claim 8, wherein the electroporation is reversible.

13. A method of carrying out irreversible electroporation of tissue, comprising:

inserting a gel into a patient by injection wherein the gel has a predetermined conductivity relative to surrounding tissue the gel being semi-solid, elastic material comprised of matter in a colloidal state;

applying current across tissue in an amount so that cells of the tissue are subjected to irreversible electroporation;

wherein the conductivity of the gel is sufficiently below that of the surrounding tissue such that when current is applied substantially no current flows through the gel and the current is directed to a specific targeted area of tissue to be irreversibly electroporated.

14. The method of claim 13, wherein the gel is comprised of a therapeutically active drug.

15. The method of claim 13, wherein the gel is a hydrogel comprised of water and solid phase polymer chains.

16. The method of claim 13, wherein the conductivity of the gel is adjusted by incorporating a predetermined concentration of ions in the gel.

17. The method of claim 16, wherein ions are derived from NaCl in the gel.

18. A method of carrying out irreversible electroporation of tissue, comprising:

inserting a gel into a patient by injection wherein the gel has a predetermined conductivity relative to surrounding tissue the gel being semi-solid, elastic material comprised of matter in a colloidal state;

applying current across tissue in an amount so that cells of the tissue are subjected to irreversible electroporation;

wherein the conductivity of the gel is substantially the same as that of surrounding tissue and when current is applied a homogeneous electrical field is applied and a specific area of targeted tissue is irreversibly electroporated.

19. A method of carrying out irreversible electroporation of tissue, comprising:

inserting a gel into tissue in a patient by injection wherein the gel has a predetermined conductivity relative to tissue surrounding the gel, wherein the gel is a semi-solid, elastic material comprised of matter in a colloidal state;

applying current across tissue in an amount so that cells of the tissue are subjected to irreversible electroporation;

wherein the conductivity of the gel is predetermined by incorporating a known concentration of ions in the gel.

20. The method of claim 19, wherein the ions are derived from NaCl dissolved in the gel and wherein the conductivity of the gel is sufficiently below that of the surrounding tissue such that when current is applied substantially no current flows through the gel and the current is directed to a specific targeted area of tissue to be irreversibly electroporated.

21. The method of claim 19, wherein the gel is a hydrogel comprised of water and solid phase polymer chains and wherein the conductivity of the gel is substantially the same as that of surrounding tissue and when current is applied a homogeneous electrical field is applied and a specific area of targeted tissue is irreversibly electroporated.

22. The method of claim 19, wherein the gel is comprised of a therapeutically active drug.