Methods, kits and compositions pertaining to combination oligomers and libraries for their preparation
First Claim
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1. A method comprising:
- reacting a first oligomer block, a second oligomer block, and optionally a condensation reagent or reagents under condensation conditions to thereby form a combination oligomer having a linker of at least three atoms in length that is not part of a nucleobase containing backbone subunit of the polymer, wherein said linker covalently links the nucleobase containing backbone subunits of the two component oligomer blocks, such that the combination oligomer hybridizes juxtaposed to a target sequence without a gap or a gap base;
wherein,(i) the first and second oligomer blocks are each independently a peptide nucleic acid oligomer, PNA chimera or PNA combination oligomer;
(ii) neither of the first nor the second oligomer block is support bound; and
(iii) the combination oligomer forms in the absence of a template.
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Abstract
This invention pertains to the field of combination oligomers, including the block synthesis of combination oligomers in the absence of a template, as well as related methods, kits, libraries and other compositions.
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Citations
31 Claims
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1. A method comprising:
- reacting a first oligomer block, a second oligomer block, and optionally a condensation reagent or reagents under condensation conditions to thereby form a combination oligomer having a linker of at least three atoms in length that is not part of a nucleobase containing backbone subunit of the polymer, wherein said linker covalently links the nucleobase containing backbone subunits of the two component oligomer blocks, such that the combination oligomer hybridizes juxtaposed to a target sequence without a gap or a gap base;
wherein,(i) the first and second oligomer blocks are each independently a peptide nucleic acid oligomer, PNA chimera or PNA combination oligomer; (ii) neither of the first nor the second oligomer block is support bound; and (iii) the combination oligomer forms in the absence of a template. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
- reacting a first oligomer block, a second oligomer block, and optionally a condensation reagent or reagents under condensation conditions to thereby form a combination oligomer having a linker of at least three atoms in length that is not part of a nucleobase containing backbone subunit of the polymer, wherein said linker covalently links the nucleobase containing backbone subunits of the two component oligomer blocks, such that the combination oligomer hybridizes juxtaposed to a target sequence without a gap or a gap base;
Specification