Compositions, probes, and conjugates and uses thereof

• US 7,674,924 B2
• Filed: 05/22/2007
• Issued: 03/09/2010
• Est. Priority Date: 05/22/2006
• Status: Active Grant

• Current Assignee: Gen-Probe Incorporated (Hologic Incorporated)
• Original Assignee: Third Wave Technologies Incorporated (Hologic Incorporated)
• Inventors: Wayland, Sarah R., Skrzypczynski, Zbigniev
• Primary Examiner(s): Riley; Jezia
• Application Number:
  US11/805,038
• Publication Number:
  US 20080071074A1
• Time in Patent Office: 1,022 Days
• Field of Search: 552/6, 536/23.1, 536/25.3, 435/116, 435/129, 514/383
• US Class Current: 552/6
• CPC Class Codes: A61K 49/0039 Coumarin dyes
• CPC Class Codes: A61K 49/0054 Macromolecular compounds, i.e. oligomers, polymers, dendrimers
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Abstract

The present invention relates to compositions useful as probes and in other applications and methods of their use. In some embodiments, nucleotides are prepared and functionalized with dyes. In some embodiments a first molecule is functionalized with an alkynyl group, a second molecule is functionalized with an azide group, and said first and second molecules are mixed under conditions to form a conjugate with a 1,2,3-triazol group. In further embodiments, a nucleotide is functionalized with an alkynyl group, a dye is functionalized with an azide group, and mixing the nucleotide and the dye forms a conjugate capable of emitting light.

Claims

1. A composition comprising a substituted or unsubstituted unreacted compound functioning to react with an azide to form a reacted compound with a 1,2,3-triazol group, said unreacted compound having the following formula: