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Method of coating fine particles with lipid film

  • US 7,678,415 B2
  • Filed: 10/04/2001
  • Issued: 03/16/2010
  • Est. Priority Date: 10/04/2000
  • Status: Expired due to Fees
First Claim
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1. A method for coating a complex of a nucleic acid and a liposome with closed vesicles of a lipid bilayer, the liposome comprising cationic lipid, the complex obtained by mixing the nucleic acid and the liposome in water, which consists of the steps of (i) and (ii):

  • (i) suspending the complex in an aqueous solution consisting essentially of;

    water,polar organic solvent(s) selected from an alcohol, a glycol and a polyalkylene glycol, andlipid(s) selected from phospholipid, glyceroglycolipid, sphingoglycolipid, cholesterol and synthetic lipid, excluding a cationic lipid,wherein the complex has a diameter of 10 nm to 1,000 nm, andwherein the lipid(s) is dissolved in the aqueous solution; and

    (ii) adding water to the aqueous solution in which the complex is suspended, so as to decrease the vol % concentration of the polar organic solvent(s) in the aqueous solution, whereby the complex is coated with closed vesicles of the lipid bilayer formed of the lipid(s).

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