Cell division marker
First Claim
Patent Images
1. A method of detecting the presence of a midbody scar in a mammalian cell, the method comprising:
- contacting a mammalian cell suspected to have a midbody scar with an antibody or antigen-binding portion thereof that binds specifically to a polypeptide component of a midbody scar selected from the group consisting of mitotic kinesin-like protein 1 (MKLP1), Epsilon tubulin (ε
-tubulin), Centrosomal Protein 55 kDa (Cep55), and Aurora B kinase;
detecting binding of the antibody to its antigen; and
identifying a midbody scar in the mammalian cell if binding of the antibody to its antigen is detected in a ring structure not at a midbody or centrosome, thereby detecting a midbody scar.
2 Assignments
0 Petitions
Accused Products
Abstract
This application relates to a newly identified animal cell structure, the midbody scar. This structure is a remnant of the midbody that is retained by one daughter cell following cytokinesis and persists through multiple subsequent cell cycles. The midbody scar can be useful as a marker of dividing cells or of a cell'"'"'s replicative age.
-
Citations
12 Claims
-
1. A method of detecting the presence of a midbody scar in a mammalian cell, the method comprising:
-
contacting a mammalian cell suspected to have a midbody scar with an antibody or antigen-binding portion thereof that binds specifically to a polypeptide component of a midbody scar selected from the group consisting of mitotic kinesin-like protein 1 (MKLP1), Epsilon tubulin (ε
-tubulin), Centrosomal Protein 55 kDa (Cep55), and Aurora B kinase;detecting binding of the antibody to its antigen; and identifying a midbody scar in the mammalian cell if binding of the antibody to its antigen is detected in a ring structure not at a midbody or centrosome, thereby detecting a midbody scar. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
-
Specification