Selective binding materials

• US 7,682,836 B2
• Filed: 10/04/2001
• Issued: 03/23/2010
• Est. Priority Date: 10/04/2000
• Status: Expired due to Fees

• Current Assignee: Cranfield University
• Original Assignee: Cranfield University
• Inventors: Karim, Khalku, Piletska, Olena Volodimirivna, Turner, Anthony Peter Francis, Piletsky, Sergiy Anatoliyovich, Bossi, Alessandra
• Primary Examiner(s): Chin; Christopher L
• Application Number:
  US10/381,593
• Publication Number:
  US 20040063112A1
• Time in Patent Office: 3,092 Days
• Field of Search: 435174-182, 435/803, 435/40.52, 436518-535, 436/161, 436/147, 436/151, 436/181, 422 681- 70, 264/28, 264219-227, 204/451, 204/462, 204/468, 204/549, 204/550, 204/551
• US Class Current: 436/524
• CPC Class Codes: B01J 20/3057 Use of a templating or imprinting material molecularly imprinted polymers B01J20/268; filling pores of a substrate or matrix followed by the removal of the substrate or matrix
• CPC Class Codes: G01N 2600/00 Assays involving molecular imprinted polymers/polymers created around a molecular template
• CPC Class Codes: G01N 33/531 Production of immunochemical test materials
• CPC Class Codes: Y10S 436/815 Test for named compound or class of compounds
• CPC Class Codes: Y10S 436/816 Alkaloids, amphetamines, and barbiturates
• CPC Class Codes: Y10S 436/817 Steroids or hormones
• CPC Class Codes: Y10S 436/818 Human chorionic gonadotropin
• CPC Class Codes: Y10S 436/901 Drugs of abuse, e.g. narcotics, amphetamine

Abstract

A template (a molecule preferably of molecular size >500 Da, or a larger entity such as a cell, virus or tissue sample) is dissolved or suspended in a fluid. The fluid is frozen, and the template is removed (e.g. by dissolution or electrophoresis, or mechanically) to leave an “imprinted” frozen fluid. This is capable of selectively adsorbing the template substance. It is usable as a separation medium, a recognition element in sensors and assays, and as a catalyst.

Claims

1. A process for forming a selective binding material comprising:

(a) preparing a composition by suspending or dissolving a template material into a carrier wherein said carrier is in a liquid state;

(b) freezing the composition to convert said carrier from a liquid state to a solid state and forming a solid composition; and

(c) at least partially removing the template material from the solid composition to leave solid carrier with binding sites at locations where the template material has been removed, to form a selective binding material, wherein

the carrier is selected from the group consisting of water, aqueous solvents, inorganic liquids, organic solvents, metals, fusible inorganic compounds, and gases.

2. The process according to claim 1 wherein in step (c) the template material is removed mechanically.

3. The process according to claim 1 wherein in step (c) the template material is removed by washing with a solvent.

4. The process according to claim 1 wherein in step (c) the template material is removed by electrodialysis.

5. The process according to claim 1, wherein the template material is selected from the group consisting of proteins, biological receptors, nucleic acids, chromosomes, cells, viruses, microorganisms, tissue samples, carbohydrates, oligosaccharides, polysaccharides, nucleoproteins, mucoproteins, lipoproteins, synthetic proteins, glycoproteins, glucosaminoglycans, steroids, immunosuppressants, hormones, heparin, antibiotics, vitamins and drugs.

6. The process according to claim 1 wherein said carrier fluid is a liquid and said composition prepared in step (a) is a solution of the template material.

7. The process according to claim 6 wherein said carrier is water and the template material is a protein.

8. The process according to claim 1, wherein the template material is immobilised onto a solid support.

9. The process of claim 1, further comprising:

(d) contacting the selective binding material with a solution comprising the template material to bind the template material at the binding sites of the selective binding material.