Systems and methods for treating patients with processed lipoaspirate cells
First Claim
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1. A self contained adipose-derived stem cell processing unit, comprising:
- a tissue collection container that is configured to receive unprocessed adipose tissue that is removed from a patient, wherein said tissue collection container is defined by a closed system;
a first filter that is disposed within said tissue collection container, wherein said first filter is configured to substantially retain a first component of said unprocessed adipose tissue and pass a second component of said unprocessed adipose tissue, such that said first filter substantially separates said first component from said second component, and wherein said first component comprises a cell population that comprises adipose-derived stem cells and said second component comprises mature adipocytes;
a cell collection container, which is configured to receive said first component comprising a cell population that comprises adipose-derived stem cells from said tissue collection container, wherein said cell collection container is within said closed system;
a conduit configured to allow passage of said first component comprising a cell population comprising adipose-derived stem cells from said tissue collection container to said cell collection container while maintaining a closed system; and
a cell concentrator disposed within said cell collection container that is configured to facilitate the concentration of said first component comprising a cell population that comprises adipose-derived stem cells, wherein said cell concentrator comprises a centrifuge or a spinning membrane filter.
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Abstract
Cells present in processed lipoaspirate tissue are used to treat patients. Methods of treating patients include processing adipose tissue to deliver a concentrated amount of stem cells obtained from the adipose tissue to a patient. The methods may be practiced in a closed system so that the stem cells are not exposed to an external environment prior to being administered to a patient. Compositions that are administered to a patient include a mixture of adipose tissue and stem cells so that the composition has a higher concentration of stem cells than when the adipose tissue was removed from the patient.
123 Citations
45 Claims
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1. A self contained adipose-derived stem cell processing unit, comprising:
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a tissue collection container that is configured to receive unprocessed adipose tissue that is removed from a patient, wherein said tissue collection container is defined by a closed system; a first filter that is disposed within said tissue collection container, wherein said first filter is configured to substantially retain a first component of said unprocessed adipose tissue and pass a second component of said unprocessed adipose tissue, such that said first filter substantially separates said first component from said second component, and wherein said first component comprises a cell population that comprises adipose-derived stem cells and said second component comprises mature adipocytes; a cell collection container, which is configured to receive said first component comprising a cell population that comprises adipose-derived stem cells from said tissue collection container, wherein said cell collection container is within said closed system; a conduit configured to allow passage of said first component comprising a cell population comprising adipose-derived stem cells from said tissue collection container to said cell collection container while maintaining a closed system; and a cell concentrator disposed within said cell collection container that is configured to facilitate the concentration of said first component comprising a cell population that comprises adipose-derived stem cells, wherein said cell concentrator comprises a centrifuge or a spinning membrane filter.
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2. A method of using the self -contained adipose-derived stem cell processing unit of claim 1 to process a cell population that comprises adipose-derived stem cells, comprising:
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removing adipose tissue that comprises a cell population that comprises adipose-derived stem cells from said patient; introducing the removed adipose tissue that comprises said cell population that comprises adipose-derived stem cells into the adipose-derived stem cell processing unit of claim 1; and separating and concentrating said cell population that comprises adipose-derived stem cells from said removed adipose tissue within said self-contained adipose-derived stem cell processing unit while maintaining said closed pathway to obtain a concentrated cell population that comprises adipose-derived stem cells.
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3. The method of claim 2, further comprising contacting said cell population that comprises adipose-derived stem cells with an additive.
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4. The method of claim 2, further comprising mixing said concentrated cell population that comprises adipose-derived stem cells with a second portion of unprocessed adipose tissue comprising intact, non-disaggregated tissue fragments from said patient.
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5. The method of claim 2, further comprising cooling said concentrated cell population that comprises adipose-derived stem cells.
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6. The method of claim 2, wherein said first portion of adipose tissue that is removed from said patient is lipoaspirate.
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7. The method of claim 2, wherein said first portion of adipose tissue that is removed from said patient is obtained by excisional lipectomy.
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8. The method of claim 4, wherein said second portion of adipose tissue that is removed from said patient is lipoaspirate.
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9. The method of claim 4, wherein said second portion of adipose tissue that is removed from said patient is obtained by excisional lipectomy.
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10. The method of claim 3, wherein said additive is a tissue or tissue fragment.
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11. The method of claim 3, wherein said additive is demineralized bone.
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12. The method of claim 3, wherein said additive is a compound of the thiaglitazone family.
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13. The method of claim 3, wherein said additive is insulin.
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14. The method of claim 3, wherein said additive is an exogenous DNA.
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15. The method of claim 3, wherein said additive is a biological or artificial scaffold.
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16. The method of claim 15, wherein said biological or artificial scaffold is a resorbable plastic sheath.
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17. The method of claim 3, wherein said additive is an immunosuppressive agent.
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18. The method of claim 17, wherein said immunosuppressive agent is selected from the group consisting of cyclosporine, myophenylate mofetil, rapamycin, and antithymocyte globulin.
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19. The method of claim 3, wherein said additive is a cell differentiation agent.
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20. The method of claim 19, wherein said cell differentiation agent is a cytokine.
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21. The method of claim 19, wherein said cell differentiation agent is a growth factor.
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22. The method of claim 3, wherein said additive is an antimicrobial agent.
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23. The method of claim 2, further comprising mechanically or enzymatically disaggregating said removed adipose tissue that comprises said cell population that comprises adipose-derived stem cells.
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24. The method of claim 2, wherein said cell population comprising said adipose-derived stem cells is separated from mature adipocytes and connective tissue present in adipose tissue that was removed from said patient by employing a filter.
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25. The adipose-derived stem cell processing unit of claim 1, wherein said cell concentrator comprises a spinning membrane filter.
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26. The adipose-derived stem cell processing unit of claim 1, wherein said cell concentrator comprises an antibody.
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27. The adipose-derived stem cell processing unit of claim 26, wherein said antibody is selected from the group consisting of AP2, CD3, CD19, and CD11 b.
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28. The adipose-derived stem cell processing unit of claim 1, wherein said cell concentrator comprises a centrifuge.
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29. The method of claim 23, wherein the removed adipose tissue that comprises said cell population that comprises adipose-derived stem cells is enzymatically digested.
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30. The method of claim 29, wherein said enzymatic digestion comprises a collagenase.
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31. The method of claim 29, wherein said enzymatic digestion comprises a neutral protease.
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32. The method of claim 29, wherein said enzymatic digestion comprises trypsin.
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33. The method of claim 2, wherein said concentrated cell population that comprises adipose-derived stem cells comprises endothelial precursor cells.
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34. The method of claim 5, wherein said cooling of said concentrated cell population that comprises adipose-derived stem cells is performed within said self-contained adipose-derived stem cell processing unit while maintaining a closed pathway.
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35. The method of claim 5, wherein said concentrated cell population that comprises adipose-derived stem cells is cryopreserved.
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36. The method of claim 3, wherein said additive is unprocessed adipose tissue that comprises intact, non-disaggregated tissue fragments.
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37. The adipose-derived stem cell processing unit of claim 1, further comprising a temperature control device.
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38. The adipose-derived stem cell processing unit of claim 37, wherein the temperature control device is configured to adjust the temperature of the components in the tissue collection container.
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39. The adipose-derived stem cell processing unit of claim 37, wherein the temperature control device is configured to adjust the temperature of fluid that is delivered to the tissue collection container.
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40. The adipose-derived stem cell processing unit of claim 1, further comprising a microprocessor configured to automate the self-contained adipose-derived stem cell processing unit.
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41. The adipose-derived stem cell processing unit of claim 40, further comprising a temperature control device.
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42. The self contained adipose-derived stem cell processing unit of claim 1, comprising an aspiration port coupled to a suction device.
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43. The self contained adipose-derived stem cell processing unit of claim 1, wherein said second component comprises lipid.
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44. The self contained adipose-derived stem cell processing unit of claim 1, wherein said second component comprises blood.
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45. The self contained adipose-derived stem cell processing unit of claim 1, further comprising an outlet configured to allow the aseptic removal of said concentrated population of cells that comprises adipose-derived stem cells.
Specification