Compositions and methods for preserving permeation layers for use on active electronic matrix devices

• US 7,687,103 B2
• Filed: 08/28/2007
• Issued: 03/30/2010
• Est. Priority Date: 08/31/2006
• Status: Expired due to Fees

• Current Assignee: Gamida for Life B.V.
• Original Assignee: Gamida for Life B.V.
• Inventors: Nguyen, Kenny V., Light, James P. II
• Primary Examiner(s): CAMERON, ERMA C
• Application Number:
  US11/846,187
• Publication Number:
  US 20080069962A1
• Time in Patent Office: 945 Days
• Field of Search: None
• US Class Current: 427/58
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001

Abstract

An improved synthetic, polymer hydrogel permeation layer for use with an active electronic matrix device for biological assays. The permeation layer includes a dried coating of raffinose or a combination of raffinose and stachyose to protect the permeation layer from degradation during shipping and storage.

Claims

1. A sugar-based coating for a permeation layer overlying an electrode on a substrate, the coating comprising a solution of raffinose and stachyose in combination.

2. The sugar-based coating of claim 1, wherein the solution of raffinose and stachyose further comprises raffinose and stachyose in combination in an amount of between about 0.5% by weight to about 10% by weight, and wherein the permeation layer comprises a material selected from the group consisting of hydrogels and sol-gels.

3. A method of coating a permeation layer overlying an electrode on a substrate, the method comprising the steps of:

a. preparing a permeation layer

b. preparing a sugar-based preservative solution comprising raffinose and stachyose in combination in an amount of between about 0.5% by weight to about 10% by weight;

c. covering the permeation layer with an amount of preservative solution sufficient to substantially cover an outside surface of the permeation layer; and

d. allowing preservative solution to dry on the permeation layer,

wherein the permeation layer comprises a material selected from the group consisting of hydrogels and sol-gels.

4. The method of claim 3, wherein the sugar-based preservative solution comprises about 5% raffinose by weight and about 5% stachyose by weight.

5. The method of claim 3, wherein the sugar-based preservative solution comprises about 9% raffinose by weight and about 1% stachyose by weight.

6. The method of claim 3, wherein the sugar-based preservative solution comprises about 0% raffinose by weight and about 10% stachyose by weight.

7. A sugar-based coating for a permeation layer overlying an electrode on a substrate, the coating comprising a solution of stachyose.

8. The sugar-based coating of claim 7, wherein the solution of stachyose further comprises stachyose in an amount of between about 0.5% by weight to about 10% by weight.