Enzyme electrode, and sensor and biofuel cell using the same

• US 7,687,186 B2
• Filed: 09/25/2006
• Issued: 03/30/2010
• Est. Priority Date: 09/30/2005
• Status: Active Grant

• Current Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: Yano, Tetsuya, Nomoto, Tsuyoshi, Kubo, Wataru
• Primary Examiner(s): RHEE, JANE J
• Application Number:
  US11/534,959
• Publication Number:
  US 20070122689A1
• Time in Patent Office: 1,282 Days
• Field of Search: 429/34, 429/43, 429/44, 204/403.01
• US Class Current: 429/401
• CPC Class Codes: H01M 4/9008 Organic or organo-metallic compounds
• CPC Class Codes: H01M 8/16 Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
• CPC Class Codes: Y02E 60/50 Fuel cells

Abstract

The present invention relates to an enzyme electrode for transporting electric charge formed by an oxidation-reduction reaction in an enzyme to a conductive member at a high speed. Specifically, the enzyme electrode has a π-conjugated metal complex molecule immobilized on the conductive member and the enzyme immobilized on the conductive member through the π-conjugated metal complex molecule.

Claims

1. An enzyme electrode comprising a conductive member and an enzyme, wherein

the conductive member has a π

-conjugated metal complex molecule immobilized thereon, and

the enzyme is immobilized on the conductive member through the π

-conjugated metal complex molecule,

wherein the π

-conjugated metal complex molecule is a polynuclear complex molecule.

2. The enzyme electrode according to claim 1, wherein the enzyme is immobilized on a ligand bonded to a metal center of the π

-conjugated metal complex molecule.

3. The enzyme electrode according to claim 1, wherein the π

-conjugated metal complex molecule has a metal center which is a transition metal element.

4. The enzyme electrode according to claim 1, wherein the π

-conjugated metal complex molecule has a bipyridine derivative or a terpyridine derivative as ligand.

5. The enzyme electrode according to claim 1, wherein the enzyme comprises a metal particle and is in the form of a composite of the enzyme and the metal particle, the metal particle being formed by reacting the enzyme with a substrate for the enzyme in the presence of a metal precursor capable of forming a metal during the reaction of the enzyme with the substrate for the enzyme.

6. The enzyme electrode according to claim 5, wherein the enzyme has at least a part of the metal particle entrapped therein and is in the form of a composite with the metal particle.

7. A sensor comprising the enzyme electrode according to claim 1 and a counter electrode.

8. A fuel cell having a region capable of holding an electrolytic solution arranged between an anode and a cathode, wherein at least one of the anode and the cathode is the enzyme electrode according to claim 1.