Bovine pregnancy test
First Claim
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1. A method for determining pregnancy of a ruminant and/or ungulate animal, the method comprising:
- (a) testing a fluid or cell derived from said animal for the presence of at least one Group A antigen selected from the group consisting of interferon-tau induced proteins, Bovine Antigen Glycoprotein (BAG), Early Pregnancy Factor (EPF), Pregnancy Specific Protein B (PSPB). and Pregnancy Associated Glycoproteins 1, 4, 5 and 9 (PAG-1, PAG-4, PAG-5, and PAG-9 or Group B antigen selected from the group consisting of estrone sulfate, pregnancy serum protein 60 (PSP-60 pregnancy-associated glycoproteins 6 and 7 (PAG-6 and PAG-7), and pregnancy-specific protein B (PSP-B);
(b) testing a fluid or cell derived from said animal for the presence of progesterone;
(c) determining that the animal is pregnant if testing is positive for the presence of at least one of said Group A or Group B antigens and progesterone; and
(d) determining that the animal is not pregnant if testing is negative for the presence of progesterone.
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Abstract
This invention provides bovine pregnancy test methods and devices. The test is also suitable for other ruminant and/or ungulate animals. Antigens from Group A (early pregnancy antigens), and/or Group B (mid-pregnancy antigens), and Group C (early, mid- and late pregnancy antigens) are detected in a fluid from the animal, and pregnancy is reliably determined. The pregnancy assays of this invention are preferably carried out using immunoassay devices which provide immediate results in the field.
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17 Claims
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1. A method for determining pregnancy of a ruminant and/or ungulate animal, the method comprising:
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(a) testing a fluid or cell derived from said animal for the presence of at least one Group A antigen selected from the group consisting of interferon-tau induced proteins, Bovine Antigen Glycoprotein (BAG), Early Pregnancy Factor (EPF), Pregnancy Specific Protein B (PSPB). and Pregnancy Associated Glycoproteins 1, 4, 5 and 9 (PAG-1, PAG-4, PAG-5, and PAG-9 or Group B antigen selected from the group consisting of estrone sulfate, pregnancy serum protein 60 (PSP-60 pregnancy-associated glycoproteins 6 and 7 (PAG-6 and PAG-7), and pregnancy-specific protein B (PSP-B); (b) testing a fluid or cell derived from said animal for the presence of progesterone; (c) determining that the animal is pregnant if testing is positive for the presence of at least one of said Group A or Group B antigens and progesterone; and (d) determining that the animal is not pregnant if testing is negative for the presence of progesterone. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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Specification