Combinatorial strategies for polymer synthesis
First Claim
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1. An automated apparatus for forming an array of ligands on a support having localized areas, comprising:
- one or more dispensers supplied with one or more solutions of a monomer or polymer,wherein the one or more dispensers is operative to dispense a volume of solution in a single coupling step of less than 5 nl at a distance away from a localized area,a support having a surface and having a density of localized areas at least about 1000 localized areas per cm2, anda positioning system having a translational controller configured to repeatedly position the dispenser relative to individual localized areas on the support with the one or more dispensers dispensing the one or more solutions of the monomer or polymer for attachment at the individual localized areas until an array of at least 100 different ligands each at individual localized areas is formed.
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Abstract
A method and device for forming large arrays of polymers on a substrate (401). According to a preferred aspect of the invention, the substrate is contacted by a channel block (407) having channels (409) therein. Selected reagents are delivered through the channels, the substrate is rotated by a rotating stage (403), and the process is repeated to form arrays of polymers on the substrate. The method may be combined with light-directed methodolgies.
181 Citations
140 Claims
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1. An automated apparatus for forming an array of ligands on a support having localized areas, comprising:
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one or more dispensers supplied with one or more solutions of a monomer or polymer, wherein the one or more dispensers is operative to dispense a volume of solution in a single coupling step of less than 5 nl at a distance away from a localized area, a support having a surface and having a density of localized areas at least about 1000 localized areas per cm2, and a positioning system having a translational controller configured to repeatedly position the dispenser relative to individual localized areas on the support with the one or more dispensers dispensing the one or more solutions of the monomer or polymer for attachment at the individual localized areas until an array of at least 100 different ligands each at individual localized areas is formed. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73, 74, 75)
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76. An automated apparatus for forming an array of ligands on a support having localized areas, comprising:
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an array of dispensing units supplied with one or more solutions of a monomer or polymer, wherein the dispensing units are operative to dispense droplets in a single coupling step of no greater than 5 nl at a distance away from a localized area, a support having a surface and having a density of localized areas at least about 1000 localized areas per cm2, and a positioning system including a translational controller capable of repeatedly positioning the dispensing units relative to individual localized areas on the support with the dispensing units dispensing the one or more solutions of the monomer or polymer for attachment at the individual localized areas until an array of at least 100 different ligands each at individual localized areas is formed. - View Dependent Claims (77, 78, 79, 80, 81, 82, 83, 84, 85, 86, 87, 88, 89, 90, 91, 92, 93, 94, 95, 96, 97, 98, 99, 100, 101, 102, 103, 104, 105, 106, 107, 108, 109, 110, 111, 112, 113, 114, 115, 116, 117, 118, 119, 120, 121, 122, 123, 124, 125, 126, 127)
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128. An automated apparatus for forming an array of polypeptides or polynucleotides on a support having localized areas, comprising:
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one or more dispensers supplied with plural solutions of polypeptides or polynucleotides, wherein the one or more dispensers is operative to dispense a volume of the plural solutions in a single coupling step of less than 5 nl at a distance away from a localized area, a support having a density of localized areas at least about 1000 localized areas per cm2, and a positioning system including a translation controller configured to repeatedly position the one or more dispensers relative to individual localized areas on the support with the one or more dispensers dispensing the plural solutions of the polypeptides or polynucleotides for attachment at the individual localized areas until an array of at least 1000 different ligands each at individual localized areas is formed. - View Dependent Claims (129, 130, 131, 132, 133, 134, 135, 136, 137, 138, 139, 140)
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Specification