Method for the fabrication of a “lab on chip” from photoresist material for medical diagnostic applications
First Claim
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1. A method for the fabrication of a device for performing immunoassays comprising:
- (a) providing a micro structured substrate having predefined indentations and/or through holes;
(b) applying at least one layer of a dry film photo resist material, wherein the dry film photo resist comprises an outer surface facing away from the substrate and an inner surface facing toward the substrate, wherein the outer surface is subject to photo lithographic light and the inner surface comprises functional chemical groups attached thereto;
(c) light exposing the dry film photo resist using a photo mask with a different predefined pattern;
(d) developing the dry film photo resist layer;
(e) repeating step (b) and step (c) using a photo mask with a different predefined pattern and step (d), so that substrate and dry film photo resist material form a capillary structure or capillary channel structure, respectively, with at least one inlet and outlet; and
(f) localized immobilizing biomolecules to at least one region of the inner surface of the dry film photo resist material channel structure generated in step (e) by chemical coupling via the functional groups of the dry film photo resist material, wherein a device for performing immuno assays is obtained.
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Abstract
The present invention relates to a device for performing immuno assays (“biochip” or “lab on chip” respectively), a procedure for its fabrication and the use of the device for performing immuno assays. Furthermore the present invention relates to the use of a photo lithographically patternable dry film photo resist based on a material with functional chemical groups for the immobilization of biomolecules.
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Citations
15 Claims
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1. A method for the fabrication of a device for performing immunoassays comprising:
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(a) providing a micro structured substrate having predefined indentations and/or through holes; (b) applying at least one layer of a dry film photo resist material, wherein the dry film photo resist comprises an outer surface facing away from the substrate and an inner surface facing toward the substrate, wherein the outer surface is subject to photo lithographic light and the inner surface comprises functional chemical groups attached thereto; (c) light exposing the dry film photo resist using a photo mask with a different predefined pattern; (d) developing the dry film photo resist layer; (e) repeating step (b) and step (c) using a photo mask with a different predefined pattern and step (d), so that substrate and dry film photo resist material form a capillary structure or capillary channel structure, respectively, with at least one inlet and outlet; and (f) localized immobilizing biomolecules to at least one region of the inner surface of the dry film photo resist material channel structure generated in step (e) by chemical coupling via the functional groups of the dry film photo resist material, wherein a device for performing immuno assays is obtained. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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