Target molecule attachment to surfaces

• US 7,691,787 B2
• Filed: 07/09/2002
• Issued: 04/06/2010
• Est. Priority Date: 09/30/1997
• Status: Expired due to Fees

• Current Assignee: Surmodics Incorporated
• Original Assignee: Surmodics Incorporated
• Inventors: Hu, Sheau-Ping, Chappa, Ralph A., Swan, Dale G., Swanson, Melvin J., Guire, Patrick E.
• Primary Examiner(s): Steele; Amber D.
• Application Number:
  US10/192,917
• Publication Number:
  US 20030148308A1
• Time in Patent Office: 2,828 Days
• Field of Search: 435/6, 435/287, 435/2, 435/174, 435/287.2, 435/287.9, 435/DIG.40, 427/2.11, 436/518, 422/68.1, 536/23.1, 536/24.3
• US Class Current: 506/32
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00531 essentially square
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/0061 The surface being organic
• CPC Class Codes: B01J 2219/00612 the surface being inorganic
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00626 Covalent
• CPC Class Codes: B01J 2219/00635 by reactive plasma treatment
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00677 Ex-situ synthesis followed by deposition on the substrate
• CPC Class Codes: B01J 2219/00711 Light-directed synthesis
• CPC Class Codes: B01J 2219/00716 Heat activated synthesis
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C07B 2200/11 Compounds covalently bound to a solid support
• CPC Class Codes: C07C 235/84 with the carbon atom of at least one of the carboxamide groups bound to a carbon atom of a six-membered aromatic ring
• CPC Class Codes: C07C 323/42 Y being a carbon atom of a six-membered aromatic ring
• CPC Class Codes: C07C 45/63 by introduction of halogen; by substitution of halogen atoms by other halogen atoms
• CPC Class Codes: C07C 49/813 : polycyclic
• CPC Class Codes: C07H 21/00 : Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: C12Q 1/6834 : Enzymatic or biochemical coupling of nucleic acids to a solid phase
• CPC Class Codes: C40B 40/06 : Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 60/14 : for creating libraries

Abstract

Method and reagent composition for covalent attachment of target molecules, such as nucleic acids, onto the surface of a substrate. The reagent composition includes groups capable of covalently binding to the target molecule. Optionally, the composition can contain photoreactive groups for use in attaching the reagent composition to the surface. The reagent composition can be used to provide activated slides for use in preparing microarrays of nucleic acids.

Claims

1. A method of preparing an activated support comprising the steps of

a) providing a support;

b) providing a reagent composition comprising a polymeric backbone having more than one pendent thermochemically reactive groups capable of forming a covalent bond with an amine or sulfhydryl functional group of a target molecule, wherein the reagent composition does not further contain a charged attracting group;

c) coupling the reagent composition to the support to form the activated support.

2. The method according to claim 1 wherein the support comprises crystalline thermoplastics or amorphous thermoplastics.

3. The method according to claim 1 wherein the polymeric backbone of the reagent composition is selected from the group consisting of acrylics, vinyls, nylons, polyurethanes, and polyethers.

4. The method according to claim 1 wherein the thermochemically reactive groups are selected from the group consisting of activated esters, epoxides, azlactones, activated hydroxyls, and maleimide.

5. The method according to claim 1 wherein the step of coupling comprises disposing the reagent composition on the support and treating the reagent composition to couple the reagent composition to the support surface.

6. The method according to claim 1 wherein the reagent composition further comprises one or more photoreactive groups pendent from the polymeric backbone of the reagent composition.

7. The method according to claim 6 wherein the photoreactive groups are photoreactive aryl ketones selected from the group consisting of acetophenone, benzophenone, anthraquinone, anthrone, and heterocyclic analogs of anthrone.

8. The method according to claim 6 wherein the step of coupling comprises treating the reagent composition with energy wherein the energy activates the one or more pendent photoreactive groups and covalently couples the reagent composition to the support surface.