Microbial fuel cell with improved anode

• US 7,695,834 B1
• Filed: 10/15/2008
• Issued: 04/13/2010
• Est. Priority Date: 10/15/2008
• Status: Active Grant

• Current Assignee: UT-Battelle LLC
• Original Assignee: UT-Battelle LLC
• Inventors: Borole, Abhijeet P.
• Primary Examiner(s): Ryan; Patrick
• Assistant Examiner(s): Thomas; Brent
• Application Number:
  US12/252,015
• Publication Number:
  US 20100092804A1
• Time in Patent Office: 545 Days
• Field of Search: 429/2
• US Class Current: 429/2
• CPC Class Codes: H01M 4/8605 Porous electrodes
• CPC Class Codes: H01M 4/92 Metals of platinum group H01M4/94 , H01M4/9058 take precedence
• CPC Class Codes: H01M 8/16 Biochemical fuel cells, i.e. cells in which microorganisms function as catalysts
• CPC Class Codes: Y02E 60/50 Fuel cells

Abstract

The present invention relates to a method for preparing a microbial fuel cell, wherein the method includes: (i) inoculating an anodic liquid medium in contact with an anode of the microbial fuel cell with one or more types of microorganisms capable of functioning by an exoelectrogenic mechanism; (ii) establishing a biofilm of the microorganisms on and/or within the anode along with a substantial absence of planktonic forms of the microorganisms by substantial removal of the planktonic microorganisms during forced flow and recirculation conditions of the anodic liquid medium; and (iii) subjecting the microorganisms of the biofilm to a growth stage by incorporating one or more carbon-containing nutritive compounds in the anodic liquid medium during biofilm formation or after biofilm formation on the anode has been established.

Claims

1. A method for preparing a microbial fuel cell, the method comprising:

inoculating an anodic liquid medium in contact with an anode of the microbial fuel cell with one or more types of microorganisms capable of functioning by an exoelectrogenic mechanism;

establishing a biofilm of said microorganisms on and/or within said anode along with a substantial absence of planktonic forms of said microorganisms by substantial removal of said planktonic microorganisms during forced flow and recirculation conditions of said anodic liquid medium; and

subjecting the microorganisms of the biofilm to a growth stage by incorporating one or more carbon-containing nutritive compounds in the anodic liquid medium during biofilm formation or after biofilm formation on the anode has been established,

wherein the anodic liquid medium is contained in an anode chamber and is separated by a cation-selective permeable material from a cathodic liquid medium in contact with a cathode, wherein the anode and cathode are in electrical communication.

2. The method of claim 1, wherein said biofilm and substantial absence of planktonic microorganisms is established by a process comprising periodic replacement of all or a portion of said anodic liquid medium.

3. The method of claim 2, wherein said anodic liquid medium is substantially replaced when an optical transmission parameter of the anodic liquid medium reaches a level indicative of the presence of planktonic microorganisms.

4. The method of claim 3, wherein the optical transmission parameter comprises optical density.

5. The method of claim 1, wherein the forced flow condition of the anodic liquid medium comprises a periodic flow rate of at least 35 mL/min.

6. The method of claim 1, wherein the forced flow condition of the anodic liquid medium comprises a continuous flow rate in the range of 2-10 mL/min, along with periodic interruption at a higher flow rate of at least 35 mL/min.

7. The method of claim 6, wherein said higher flow rate is at least 40 mL/min.

8. The method of claim 6, wherein said higher flow rate is at least 45 mL/min.

9. The method of claim 6, wherein said higher flow rate is at least 50 mL/min.

10. The method of claim 1, wherein said microorganisms comprise bacteria.

11. The method of claim 1, wherein said anode has a volume and the anode chamber has a volume, wherein a ratio of the volume of the anode to the volume of the anode chamber is at least 0.5.

12. The method of claim 11, wherein the ratio of the volume of the anode to the volume of the anode chamber is at least 0.8.

13. The method of claim 11, wherein the ratio of the volume of the anode to the volume of the anode chamber is at least 0.9.

14. The method of claim 11, wherein the ratio of the volume of the anode to the volume of the anode chamber is approximately 1, thereby attaining an essentially zero dead volume in the anode chamber.

15. The method of claim 1, wherein said anode comprises a form of elemental carbon.

16. The method of claim 15, wherein said anode comprises carbon fiber, carbon paper, carbon foam, carbon cloth, carbon felt, carbon wool, carbon granules, carbon brushes, or graphite.

17. The method of claim 1, wherein the cathode of said microbial fuel cell is a Pt/air electrode.

18. The method from claim 1, wherein said growth stage is achieved by incorporating one or more carbon-containing nutritive compounds in the anodic liquid medium after biofilm formation on the anode along with a substantial absence of planktonic forms of the microorganisms has been established.

19. The method from claim 1, wherein said growth stage is achieved by incorporating one or more carbon-containing nutritive compounds in the anodic liquid medium during the time that the biofilm is being established on the anode.

20. The method of claim 1, wherein said one or more carbon-containing nutritive compounds comprise one or more carboxylic acid-containing compounds and/or their salts.

21. The method of claim 1, wherein said one or more carbon-containing nutritive compounds comprise one or more monosaccharides, disaccharides, polysaccharides, or a combination thereof.

22. The method of claim 1, wherein the growth stage is continued until an electrical current output of the microbial fuel cell becomes level at a fixed resistance between the anode and cathode.

23. The method of claim 1, further comprising a starvation stage of the microorganisms after said growth stage of microorganisms, wherein carbon-containing nutritive compounds present in the anodic liquid medium are reduced below an amount necessary for the microorganisms to produce a maximum achievable current under conditions provided by the microbial fuel cell, for a period of time that permits enrichment of the biofilm with microorganisms capable of storing carbon internally.

24. The method of claim 1, further comprising reducing the electrical resistance of the microbial fuel cell in order to operate the microbial fuel cell at an increased electrical current output for a period of time sufficient to further enrich the biofilm with microorganisms capable of functioning by an exoelectrogenic mechanism.

25. The method of claim 1, further comprising reducing the electrical resistance of the microbial fuel cell in order to operate the microbial fuel cell at an increased electrical current output for a period of time sufficient to further enrich the biofilm with microorganisms capable of functioning by an exoelectrogenic mechanism.

26. The method of claim 23, further comprising reducing the electrical resistance of the microbial fuel cell in order to operate the microbial fuel cell at an increased electrical current output for a period of time sufficient to further enrich the biofilm with microorganisms capable of functioning by an exoelectrogenic mechanism.

30. A method for generating electricity, the method comprising operating a microbial fuel cell that has been prepared according to claim 1.

31. A method for generating electricity, the method comprising operating a microbial fuel cell that has been prepared according to claim 18.

32. A method for generating electricity, the method comprising operating a microbial fuel cell that has been prepared according to claim 23.

33. A method for generating electricity, the method comprising operating a microbial fuel cell that has been prepared according to claim 26.

36. A method for generating hydrogen gas, the method comprising operating a microbial fuel cell that has been prepared according to claim 1, except that said anodic and cathodic liquid media are optionally not separated, wherein operation of the microbial fuel cell is conducted under the condition that the cathode is constructed of a hydrogen-producing material and is deoxygenated, and adjusting the cell potential of the microbial fuel cell by application of an external voltage such that hydrogen is produced at the cathode.

37. A method for generating hydrogen gas, the method comprising operating a microbial fuel cell that has been prepared according to claim 23, except that said anodic and cathodic liquid media are optionally not separated, wherein operation of the microbial fuel cell is conducted under the condition that the cathode is constructed of a hydrogen-producing material and is deoxygenated, and adjusting the cell potential of the microbial fuel cell by application of an external voltage such that hydrogen is produced at the cathode.

38. A method for generating hydrogen gas, the method comprising operating a microbial fuel cell that has been prepared according to claim 26, except that said anodic and cathodic liquid media are optionally not separated, wherein operation of the microbial fuel cell is conducted under the condition that the cathode is constructed of a hydrogen-producing material and is deoxygenated, and adjusting the cell potential of the microbial fuel cell by application of an external voltage such that hydrogen is produced at the cathode.

41. The method according to claim 36, wherein the cell potential is adjusted to at least 0.41 volts.

42. A microbial fuel cell prepared by the method of any of claims 1, 27, or 28.

45. The method of claim 23, wherein carbon-containing nutritive compounds are substantially eliminated from the anodic liquid medium.

46. A method for reductively processing one or more electrochemically reducible species, the method comprising operating a microbial fuel cell that has been prepared according to claim 1, wherein operation of the microbial fuel cell is conducted under the condition that the cathode is in contact with said electrochemically reducible species.

47. The method of claim 46, wherein said electrochemically reducible species is a nitrate-containing compound or material.

48. The method of claim 46, wherein said electrochemically reducible species is a perchlorate-containing compound or material.

49. The method of claim 46, wherein said electrochemically reducible species is a metal-containing compound or material.

50. The method of claim 1, wherein in said method, there is a substantial absence of a mediator substance.

52. The method of claim 1, wherein the forced flow condition renders microorganisms which do not have a propensity for forming a biofilm waterborne to enable their removal.

53. The method of claim 1, wherein the biofilm contains a population of microorganisms enriched in microorganisms which contain cellular structures for binding to the anode and each other.

27. A method for preparing a microbial fuel cell, the method comprising:

(i) inoculating an anodic liquid medium in contact with an anode of the microbial fuel cell with one or more types of microorganisms capable of functioning by an exoelectrogenic mechanism; and

(ii) establishing a biofilm of said microorganisms on and/or within said anode along with a substantial absence of planktonic forms of said microorganisms by substantial removal of said planktonic microorganisms during forced flow and recirculation conditions of said anodic liquid medium;

(iii) subjecting the microorganisms of the biofilm to a growth stage by incorporating one or more carbon-containing nutritive compounds in the anodic liquid medium during or after biofilm formation on the anode has been established; and

(iv) subjecting the microorganisms of the biofilm to a starvation stage after said growth stage by substantially eliminating carbon-containing nutritive compounds from the anodic liquid medium for a period of time in which enrichment of the biofilm with microorganisms capable of storing carbon internally is achieved,

wherein the anodic liquid medium is contained in an anode chamber and is separated by a cation-selective permeable material from a cathodic liquid medium in contact with a cathode, wherein the anode and cathode are in electrical communication.

34. A method for generating electricity, the method comprising operating a microbial fuel cell that has been prepared according to claim 27.

39. A method for generating hydrogen gas, the method comprising operating a microbial fuel cell that has been prepared according to claim 27, except that said anodic and cathodic liquid media are optionally not separated, wherein operation of the microbial fuel cell is conducted under the condition that the cathode is constructed of a hydrogen-producing material and is deoxygenated, and adjusting the cell potential of the microbial fuel cell by application of an external voltage such that hydrogen is produced at the cathode.

28. A method for preparing a microbial fuel cell, the method comprising:

(i) inoculating an anodic liquid medium in contact with an anode of the microbial fuel cell with one or more types of microorganisms capable of functioning by an exoelectrogenic mechanism; and

(ii) establishing a biofilm of said microorganisms on and/or within said anode along with a substantial absence of planktonic forms of said microorganisms by substantial removal of said planktonic microorganisms during forced flow and recirculation conditions of said anodic liquid medium;

(iii) subjecting the microorganisms of the biofilm to a growth stage by incorporating one or more carbon-containing nutritive compounds in the anodic liquid medium during or after biofilm formation on the anode has been established;

(iv) subjecting the microorganisms of the biofilm to a starvation stage after said growth stage by substantially eliminating carbon-containing nutritive compounds from the anodic liquid medium for a period of time in which enrichment of the biofilm with microorganisms capable of storing carbon internally is achieved; and

(v) reducing the electrical resistance of the microbial fuel cell in order to operate the microbial fuel cell at an increased electrical current output for a period of time sufficient to further enrich the biofilm with microorganisms capable of functioning by an exoelectrogenic mechanism,

wherein the anodic liquid medium is contained in an anode chamber and is separated by a cation-selective permeable material from a cathodic liquid medium in contact with a cathode, wherein the anode and cathode are in electrical communication.

29. The method of claim 28, wherein the step of reducing the electrical resistance of the microbial fuel cell is performed after said starvation stage.

35. A method for generating electricity, the method comprising operating a microbial fuel cell that has been prepared according to claim 28.

40. A method for generating hydrogen gas, the method comprising operating a microbial fuel cell that has been prepared according to claim 28, wherein operation of the microbial fuel cell is conducted under the condition that the cathode is constructed of a hydrogen-producing material and is deoxygenated, and adjusting the cell potential of the microbial fuel cell by application of an external voltage such that hydrogen is produced at the cathode.

43. A microbial fuel cell characterized by the presence of a biofilm of exoelectrogenic microorganisms on an anode of said microbial fuel cell, wherein said biofilm containing contains a portion of microorganisms capable of storing carbonaceous compounds or materials internally, and said biofilm is further characterized by a substantial absence of microorganisms possessing a planktonic tendency, wherein said anode is in electrical communication with a cathode, and said anode and cathode are in contact with, respectively, anodic and cathodic solutions that are separated by a cation-selective permeable membrane, and wherein said anode has a volume, and in anode chamber containing the anode has a volume, wherein a ratio of the volume of the anode to the volume of the anode chamber is at least 0.5, and wherein said substantial absence of microorganisms possessing a planktonic tendency has been achieved by forced flow and recirculation of said anodic solution.

44. The microbial fuel cell of claim 43, wherein the ratio of the volume of the anode to the volume of the anode chamber is approximately 1, thereby attaining an essentially zero dead volume in the anode chamber.

51. The microbial fuel cell of claim 43, wherein in said microbial fuel cell, there is a substantial absence of a mediator substance.