Device for cell separation and analysis and method of using

• US 7,695,956 B2
• Filed: 01/12/2006
• Issued: 04/13/2010
• Est. Priority Date: 01/12/2006
• Status: Active Grant

• Current Assignee: Biocept, Inc.
• Original Assignee: Biocept, Inc.
• Inventors: Tsinberg, Pavel, Tang, Zhongliang
• Primary Examiner(s): Yu; Melanie J.
• Application Number:
  US11/331,988
• Publication Number:
  US 20070161051A1
• Time in Patent Office: 1,552 Days
• Field of Search: 435/7.1, 435/283.1, 435/287.1, 435/287.2, 435/288.7, 435/288.5, 436/518, 422/50, 422/61, 422/68.1
• US Class Current: 435/288.5
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: G01N 1/2813 Producing thin layers of samples on a substrate, e.g. smearing, spinning-on G01N1/30 takes precedence
• CPC Class Codes: G01N 1/40 Concentrating samples

Abstract

A microflow device for separating or isolating cells from a bodily fluid or other liquid sample uses a flow path where straight-line flow is interrupted by a pattern of transverse posts which are arranged across the width of a collection region in an irregular or set random pattern so as to disrupt streamlined flow. Sequestering agents, such as Abs, are attached to all surfaces in the collection region via a hydrophilic permeable hydrogel coating. The collection region is formed as a cavity in a body molded from PDMS, which flexible body is sandwiched between a glass slide or comparable flat plate and a rigid top cap plate, both of which are pressed into abutting relation with the PDMS body by a heat-shrunk polymeric sleeve. Following cell separation and washing, cells can be released from the sequestering agents and the device centrifuged to force said cells to collect adjacent the hydrogel-coated slide or plate. Slitting the polymeric sleeve allows the body to then be peeled from the slide or plate, using an integral tab, to expose the separated cells on the top surface thereof for ready microscopic examination.

Claims

1. A microflow device comprising:

a body having a randomized flow path which comprises an inlet, an outlet, and a microchannel arrangement extending between said inlet and outlet, wherein said microchannel arrangement is housed in a cavity in a surface of said body and comprises a plurality of transverse separator posts integral with a base surface of said cavity and projecting therefrom, wherein said posts are arranged in a random and irregular pattern;

a closure plate having a top surface that is in contact with the surface of said body so that said cavity is closed; and

a polymeric wrap encircling said body and said closure plate.

2. The device of claim 1, wherein said body is molded from a flexible, optically transparent polymeric material.

3. The device of claim 2, wherein said polymeric material is selected from the group consisting of polydimethylsiloxane (PDMS), polymethylmethacrylate (PMMA), polycarbonate, polystyrene, and polyethylene teraphthalate.

4. The device of claim 1, wherein said body is molded with a tab extending from one longitudinal end thereof.

5. The device of claim 1, wherein the width of said closure plate is wider than the width of said body.

6. The device of claim 1, further comprising a cap superimposed atop said body so that said encircling polymeric wrap sandwiches said body between said cap and said closure plate.

7. The device of claim 6, wherein said cap has longitudinal sides that are beveled.

8. The device of claim 6, wherein said cap has a width, at its widest dimension, equal to between about 75% and 100% of said body.

9. The device of claim 1, wherein said polymeric wrap is a heatshrunken sleeve of polymeric material.

10. The device of claim 1, wherein said polymeric wrap can be cut to allow separation of said body from said closure plate.

11. The device of claim 1, wherein said inlet and said outlet comprise passageways having axes which are aligned at between 120°

and 150°

to said flat bottom surface of said body.

12. The device of claim 11, wherein said axes lie in a vertical plane substantially perpendicular to said flat bottom surface of said body and are angularly aligned at between 80°

and 100°

to each other.

13. The device of claim 1, wherein said inlet comprises a well capable of holding a liquid sample.

14. The device of claim 1, wherein said posts are substantially perpendicular to the base surface of said cavity.

15. The device of claim 1, wherein said posts extend to the top surface of said closure plate.

16. The device of claim 1, wherein said closure plate is affixed to distal ends of said posts.

17. The device of claim 1, wherein said posts have at least about 3 different cross sectional sizes.

18. The device of claim 1, wherein said posts are between about 70 microns to about 130 microns in diameter.

19. The device of claim 1, wherein the minimum separation between said posts is about 50 μ

M.

20. The device of claim 1, wherein the total volume of said posts occupies about 15% to 25% of the total volume of said microchannel.

21. The device of claim 1, wherein the randomized flow path provided by said posts disrupts stream-lined flow in said microchannel.

22. The device of claim 1, wherein the randomized flow path provided by said posts prevents straight-line flow in said microchannel.

23. The device of claim 1, wherein the surface of said microchannel is derivatized or coated to facilitate the attachment of a sequestering agent.

24. The device of claim 1, wherein the surface of said microchannel is coated with a layer of hydrogel at least about 1 micron thick.

25. The device of claim 24, wherein said hydrogel comprises an isocyanate-functional prepolymer comprising PEG, PPG, or a copolymer thereof.

26. The device of claim 1, wherein a sequestering agent is directly or indirectly attached to the surface of said microchannel.

27. The device of claim 1, wherein a sequestering agent is attached to the surface of said microchannel via a hydrophilic linker or a layer of hydrogel.

28. The device of claim 1, wherein the surface of said microchannel is coated with a sequestering agent selected from the group consisting of antibody, antigen, receptor, ligand, oligonucleotide, and peptide.

29. The device of claim 1, wherein said closure plate is optically transparent.

30. A method of capturing a target biomolecule comprising causing a sample containing a target biomolecule to flow through said microchannel of the device of claim 1, wherein the surface of said microchannel is coated with a sequestering agent capable of binding to said target biomolecule.

31. The method of claim 30, wherein the sample flows through said microchannel at a rate of about 0.1 mm to about 2 mm per second.

32. The method of claim 30, wherein the sample flows through said microchannel at a rate of about 0.2 mm to about 1 mm per second.

33. A method of detecting a target biomolecule in a sample comprising:

causing a sample to flow through said microchannel of the device of claim 1, wherein the surface of said microchannel is coated with a sequestering agent capable of binding to the target biomolecule; and

detecting the target biomolecule on the surface of said microchannel.

34. The method of claim 33, wherein the target molecule is a cell.

35. The method of claim 34, wherein the cell is a fetal cell.

36. The method of claim 34, wherein the cell is a cancer cell or a tumor cell.

37. The method of claim 33, wherein said sample comprises blood or cervical mucous.

38. The method of claim 33, wherein the target molecule is a virus, polynucleotide, protein, or carbohydrate.

39. The method of claim 33, wherein the sample flows through said microchannel at a rate of about 0.1 mm to about 2 mm per second.

40. The method of claim 33, wherein the sample flows through said microchannel at a rate of about 0.2 mm to about 1 mm per second.

41. The device of claim 1, wherein the spacing between said posts is variable.

42. The device of claim 1, wherein the microchannel arrangement comprises a collection region, and wherein said posts are arranged in a random and irregular pattern across the entire width of said collection region.