Methods and apparatus for analysis of phenotypic parameters and traits in plants
First Claim
1. A method for the rapid analysis of a selected phenotypic parameter in a plant, the method comprising:
- a) providing a population of plants grown in an environmentally controlled greenhouse environment;
b) providing a light spectrum digital analyzing device;
c) using the light spectrum digital analyzing device to take original reflected light images of each plant and then analyze those images for the phenotypic parameter in each plant;
d) comparing the phenotypic parameter between at least two plants; and
e) selecting a plant with the selected phenotypic parameter from a population of plants with different genetic constitution,wherein each plant within the population of plants is associated with a machine-readable identifier which can convey information about the plant'"'"'s identity and location.
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Abstract
One aspect of the invention relates to methods for analysis of phenotypic parameters or a trait in a plant comprising using a digital analyzing device, comparing phenotypic parameters or traits between plants in a population, and selecting plants with a selected parameter or trait. The invention also relates to an apparatus suitable for use in conjunction with a container in which one or more plants is growing and having associated with it a device which comprises a unique identifier.
The apparatus may comprise a transporter system for moving the containers, and may comprise a workstation at which an operation, such as imaging, is performed on plants in the containers. The operation may be performed automatically, and/or at a high throughput rate. The unique identifier of each container and the information derived from each plant may be linked to this identifier via a computer, and may be stored in a digital database.
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Citations
19 Claims
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1. A method for the rapid analysis of a selected phenotypic parameter in a plant, the method comprising:
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a) providing a population of plants grown in an environmentally controlled greenhouse environment; b) providing a light spectrum digital analyzing device; c) using the light spectrum digital analyzing device to take original reflected light images of each plant and then analyze those images for the phenotypic parameter in each plant; d) comparing the phenotypic parameter between at least two plants; and e) selecting a plant with the selected phenotypic parameter from a population of plants with different genetic constitution, wherein each plant within the population of plants is associated with a machine-readable identifier which can convey information about the plant'"'"'s identity and location. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. A method for the high throughput analysis of a trait in a plant, the method comprising:
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a) providing plants for breeding purposes with different genetic constitution; b) allowing the plants to grow in an environmentally controlled greenhouse environment, each plant being associated with a machine-readable label that distinguishes the plant from other plants; c) analyzing each plant for the trait using quantitative, non-destructive imaging technology and associating the trait with the machine-readable information number for that plant; and d) comparing the trait for one or more plants with the trait of the population of plants to determine the effect of genetic constitution on the trait. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 19)
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Specification