Non-evasive method and apparatus of detection of organism tissues

• US 7,697,976 B2
• Filed: 01/18/2005
• Issued: 04/13/2010
• Est. Priority Date: 01/19/2004
• Status: Expired due to Fees

• Current Assignee: Beijing Second Optical Instrumentfacotry, Peking University
• Original Assignee: Beijing Second Optical Instrumentfacotry, Peking University
• Inventors: Xu, Zhi, Liu, Yufeng, Liu, Zhi, Wang, Lixin, Peng, Xin, Zhang, Nengwei, Sun, Xuejun, Zhang, Yuanfu, Yu, Guangyan, Sun, Kaihua, Jiang, Jianxiong, Weng, Shifu, Wang, Bingbing, Ling, Xiaofeng, Yang, Zhanlan, Lai, Guoqiao, Yang, Limin, Wu, Jinguang, Li, Qingbo, Wu, Huizhong, Pan, Qinghua, Li, Weihong, Gong, Rongye, Zhou, Weijin, Zhou, Su, Gao, Xuejun, Xu, Duanfu, Xu, Yizhuang, Wu, Qiguang, Zhang, Li, Zhao, Ying
• Primary Examiner(s): Winakur; Eric F
• Assistant Examiner(s): Brutus; Joel F
• Application Number:
  US10/586,272
• Publication Number:
  US 20080228083A1
• Time in Patent Office: 1,911 Days
• Field of Search: 600/473, 600/476, 600/478
• US Class Current: 600/473
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/6848 Needles
• CPC Class Codes: A61B 5/7257 using Fourier transforms

Abstract

The invention relates to a non-invasive method to diagnose the changes of molecular structures of organism tissues from body surface and a dedicated apparatus. The apparatus is comprised of a Fourier Transform infrared spectrometer and a set of additional accessories. Said additional accessories include a mid-IR fiber optics sampling attachment, a fiber coupling part, and an infrared detector part. The detection method is comprised of placing the ATR probe of the dedicated apparatus on the skin surface of a region to be tested, and scanning more than one time in which the resolution of the apparatus is 1-32 cm⁻¹ and the range of the spectrum is 800-4000 cm⁻¹. It is possible to detect changes in molecular structures of living biological tissues in the early stages of cancer, and testees will not feel uncomfortable during testing. The method is easy to operate, quick, accurate, and it doesn'"'"'t harm the body.

Claims

1. A dedicated apparatus for detecting changes in molecular structure of human body surface tissue and diagnosing the properties and the degree of pathologic change of a gland tumor in a corresponding region, the apparatus comprising:

a Fourier Transform mid-infrared spectrometer, used in conjunction with a mid-infrared fiber optics sampling attachment to acquire mid-infrared light reflected from human body surface tissue, wherein the Fourier Transform mid-infrared spectrometer includes;

an interferometer part for generating mid-infrared light to be provided to the mid-infrared fiber optics sampling attachment;

an infrared detector part comprising an abaxial parabolic mirror, a detector and a 3-dimensional tuning holder, for maximizing the collection of the mid-infrared light acquired by an ATR probe of the mid-infrared fiber sampling attachment, and for generating mid-infrared spectral data; and

a data processor for processing the mid-infrared spectral data and comparing the processed infrared spectral data with a tumor database to diagnose the properties and the degree of pathologic change of the tested gland tumor;

a mid-infrared fiber sampling attachment, connected to a fiber coupling part, wherein the mid-infrared fiber sampling attachment includes a mid-infrared incident fiber, a mid-infrared exiting fiber, and a ZnSe or Ge ATR probe, each of the mid-infrared incident and exiting fibers is a hollow fiber with multiple layers of different coatings on its inside wall, and the ATR probe is of a tapered shape or cylindered shape with an inclined section and is able to acquire an subcutaneous infrared spectrum of the tumor at the body surface when being placed on and in tight contact with the body surface;

a fiber coupling part, placed between the interferometer part and the infrared detector part of the Fourier Transform infrared spectrometer, wherein the fiber sampling attachment is fixed onto the fiber coupling part, and the fiber coupling part includes two pieces of abaxial parabolic mirrors, as well as reflection mirrors and a precise fine-tuning mechanism for adjusting the parabolic mirrors, the fiber coupling part makes parallel light from the interferometer part converge into a point with a diameter of 1-3 mm so as to couple the converged light into the mid-infrared incident fiber of the fiber sampling attachment, and couples light from the mid-infrared exiting fiber of the fiber sampling attachment into the infrared detector part.

2. The dedicated apparatus as described in claim 1, wherein the Fourier Transform mid-infrared spectrometer uses ZnSe as infrared window material, each part allowing infrared transmission is made of ZnSe.

3. The dedicated apparatus as described in claim 1, wherein the tumor database stores spectral data criterion for diagnosing whether a gland tissue has pathologic changes or not, and is established based on statistical analysis on infrared spectrum data of the tissue of a certain number of healthy persons and patients.

4. The dedicated apparatus as described in claim 1, wherein the mid-infrared incident and exiting fibers used in the fiber sampling attachment have a diameter of 1-3 mm, and a transmitting range of 700-4000 cm⁻

1.

5. The dedicated apparatus as described in claim 1, wherein for diagnosis of pathologic changes in the mammary glands, the band widths, peak positions, peak intensities/peak areas, and relative intensity variations of the peaks in the region of 1000 cm⁻

1-1800 cm<sup>−

1</sup> and 2800 cm<sup>−

1</sup>-3000 cm<sup>−

1</sup> are detected.

6. The dedicated apparatus as described in claim 5, wherein for diagnosis of pathologic changes in the mammary glands, the band widths, peak positions, peak intensities/peak areas, and relative intensity variations of the peaks in the region of 1400 cm⁻

1-1490 cm<sup>−

1</sup> are detected.

7. The dedicated apparatus as described in claim 6, wherein for diagnosis of pathologic changes in the mammary glands, variations in band widths, peak positions, and peak intensities/peak areas of 1460 cm⁻

1 and 1400 cm<sup>−

1</sup> bands are detected.

8. The dedicated apparatus as described in claim 1, wherein for diagnosis of pathologic changes in the thyroid glands, the band widths, peak positions, peak intensities/peak areas, and relative intensity variations of the peaks in the region of 2800 cm⁻

1-3000 cm<sup>−

1</sup> and 1000 cm<sup>−

1</sup>-1800 cm<sup>−

1</sup> are detected.

9. The dedicated apparatus as described in claim 8, wherein for diagnosis of pathologic changes in the thyroid glands, the band widths, peak positions, peak intensities/peak areas, and relative intensity variations of the peaks in the region of 2800 cm⁻

1-3000 cm<sup>−

1</sup> and 1400 cm<sup>−

1</sup>-1500 cm<sup>−

1</sup> are detected.

10. The dedicated apparatus as described in claim 8, wherein for diagnosis of pathologic changes in the thyroid glands, the band widths, peak positions, peak intensities/peak areas, and relative intensity variations of the peaks in the region of 1200 cm⁻

1-1580 cm<sup>−

1</sup> are detected.

11. The dedicated apparatus as described in claim 1, wherein for diagnosis of pathologic changes in the parotid and submaxillary glands, the band widths, peak positions, peak intensities/peak areas, and relative intensity variations of the peaks in the region of 1000 cm⁻

1-1800 cm<sup>−

1</sup> are detected.

12. The dedicated apparatus as described in claim 11, wherein for diagnosis of pathologic changes in the parotid and submaxillary glands, the band widths, peak positions, peak intensities/peak areas, and relative intensity variations of the peaks in the region of 1200 cm⁻

1-1580 cm<sup>−

1</sup> are detected.

13. A method for diagnosing the properties and the degree of pathologic change of a gland tumor in a corresponding region by detecting changes of molecular structure of human body surface tissue comprising of the following steps:

(1) providing the apparatus as described in claim 1, and selecting operation parameters of the apparatus for detecting the related glands;

(2) cleaning and sterilizing the ATR probe and the skin of a human body surface to be tested;

(3) switching on the apparatus, then scanning and recording a spectrum of air acquired by the ATR probe for being used as a background spectrum;

(4) placing the ATR probe on the skin surface of the region to be tested, the ATR probe being in tight contact with the skin, then using the apparatus to scan and record a spectrum of the cleaned human body surface;

(5) comparing the recorded spectrum with tumor data in the tumor database to diagnose the properties and the degree of pathologic change of the gland tumor.

14. The method as described in claim 13, wherein for diagnosis of pathologic changes in the mammary glands, the band widths, peak positions, peak intensities/peak areas, and relative intensity variations of the peaks in the region of 1000 cm⁻

1-1800 cm<sup>−

1</sup> and 2800 cm<sup>−

1</sup>-3000 cm<sup>−

1</sup> are detected.

15. The method as described in claim 14, wherein for diagnosis of pathologic changes in the mammary glands, the band widths, peak positions, peak intensities/peak areas, and relative intensity variations of the peaks in the region of 1400 cm⁻

1-1490 cm<sup>−

1</sup> are detected.

16. The method as described in claim 15, wherein for diagnosis of pathologic changes in the mammary glands, variations in band widths, peak positions, peak intensities/peak areas of 1460 cm⁻

1 and 1400 cm<sup>−

1</sup> are detected.

17. The method as described in claim 13, wherein for diagnosis of pathologic changes in the thyroid glands, the band widths, peak positions, peak intensities/peak areas, and relative intensity variations of the peaks in the region of 2800 cm⁻

1-3000 cm<sup>−

1</sup> and 1000 cm<sup>−

1</sup>-1800 cm<sup>−

1</sup> are detected.

18. The method as described in claim 17, wherein for diagnosis of pathologic changes in the thyroid glands, the band widths, peak positions, peak intensities/peak areas, and relative intensity variations of the peaks in the region of 2800 cm⁻

1-3000 cm<sup>−

1</sup> and 1400 cm<sup>−

1</sup>-1500 cm<sup>−

1</sup> are detected.

19. The method as described in claim 18, wherein for diagnosis of pathologic changes in the thyroid glands, the band widths, peak positions, peak intensities/peak areas, and relative intensity variations of the peaks in the region of 1200 cm⁻

1-1580 cm<sup>−

1</sup> are detected.

20. The method as described in claim 13, wherein for diagnosis of pathologic changes in the parotid and submaxillary glands, the band widths, peak positions, peak intensities/peak areas, and relative intensity variations of the peaks in the region of 1000 cm⁻

1-1800 cm<sup>−

1</sup> are detected.

21. The method as described in claim 20, wherein for diagnosis of pathologic changes in the parotid and submaxillary glands, the band widths, peak positions, peak intensities/peak areas, and relative intensity variations of the peaks in the region of 1200 cm⁻

1-1580 cm<sup>−

1</sup> are detected.