Multiple laminar flow-based particle and cellular separation with laser steering
First Claim
1. A method of sorting a mixture comprising:
- providing an input solution, including a plurality of components, through an input channel into a sorting region as a substantially laminar first flow;
providing a buffer solution through a buffer channel into said sorting region as a substantially laminar second flow;
contacting said first flow with said second flow in said sorting region, said first flow and said second flow having a substantially non-turbulent, laminar flow separation interface within said sorting region; and
sorting said plurality of components from said input channel in said sorting region using holographic optical trapping, such that selected components from said plurality of components are optically trapped and removed from said sorting region via a selected channel into a selected solution, and unselected components from said plurality of components are removed from said sorting region via a waste channel.
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Abstract
The invention provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.
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Citations
29 Claims
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1. A method of sorting a mixture comprising:
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providing an input solution, including a plurality of components, through an input channel into a sorting region as a substantially laminar first flow; providing a buffer solution through a buffer channel into said sorting region as a substantially laminar second flow; contacting said first flow with said second flow in said sorting region, said first flow and said second flow having a substantially non-turbulent, laminar flow separation interface within said sorting region; and sorting said plurality of components from said input channel in said sorting region using holographic optical trapping, such that selected components from said plurality of components are optically trapped and removed from said sorting region via a selected channel into a selected solution, and unselected components from said plurality of components are removed from said sorting region via a waste channel. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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10. An apparatus for sorting a mixture, comprising:
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an input reservoir which holds an input solution including a plurality of components; a buffer reservoir which holds a buffer solution; a sorter having an input channel for input of said input solution, and a buffer channel for input of said buffer solution, and a selection channel for removal of selected components of said plurality of components, and a waste channel for removal of unselected components of said plurality of components; wherein said input solution and said buffer solution enter said sorter as a first flow and second flow, respectively, as substantially laminar flows, and said first flow and said second flow contact each other in a sorting region of said sorter, said first flow and said second flow having a substantially non-turbulent, laminar flow separation interface within said sorting region; and a holographic optical trapping system which optically traps said selected components of said plurality of components in said sorting region, and moves said selected components to said selection channel, whereas unselected components exit through said waste channel. - View Dependent Claims (11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29)
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Specification