Stabilization of enzymes in an emulsion by cross-linking
First Claim
1. A process for producing enzyme particles comprising:
- (a) providing an emulsion of droplets of a first liquid phase dispersed in a second liquid phase, with the one liquid phase being a hydrophilic phase and the other liquid phase being a hydrophobic phase which is immiscible with the hydrophilic phase, and with enzyme molecules being located at or within interfacial boundaries of the droplets and the second liquid phase;
(b) adding a temporary protectant to the hydrophilic phase and/or to the hydrophobic phase and/or to the emulsion, wherein the temporary protectant occupies active sites of the enzyme, thereby inhibiting occupation of the active sites by a cross-linking agent or reaction of a cross-linking agent with the active sites;
(c) adding a cross-linking agent to the hydrophilic phase and/or to the hydrophobic phase and/or to the emulsion;
(d) cross-linking, by means of the cross-linking agent, the enzyme molecules of the respective droplets; and
(e) recovering the individual enzyme particles from the second liquid phase.
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Abstract
A process for producing enzymes structures includes providing an emulsion of droplets of a first liquid phase dispersed in a second liquid phase. The one liquid phase is a hydrophilic phase, while the other liquid phase is a hydrophobic phase which is immiscible with the hydrophilic phase. Enzyme molecules are located at or within interfacial boundaries of the droplets and the second liquid phase. The enzyme molecules of the respective droplets are cross-linked so that individual enzyme structures, which are stable and in which the enzymes are immobilized with a majority of active sites of the enzymes being orientated either internally or externally, are formed from individual droplets.
5 Citations
18 Claims
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1. A process for producing enzyme particles comprising:
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(a) providing an emulsion of droplets of a first liquid phase dispersed in a second liquid phase, with the one liquid phase being a hydrophilic phase and the other liquid phase being a hydrophobic phase which is immiscible with the hydrophilic phase, and with enzyme molecules being located at or within interfacial boundaries of the droplets and the second liquid phase; (b) adding a temporary protectant to the hydrophilic phase and/or to the hydrophobic phase and/or to the emulsion, wherein the temporary protectant occupies active sites of the enzyme, thereby inhibiting occupation of the active sites by a cross-linking agent or reaction of a cross-linking agent with the active sites; (c) adding a cross-linking agent to the hydrophilic phase and/or to the hydrophobic phase and/or to the emulsion; (d) cross-linking, by means of the cross-linking agent, the enzyme molecules of the respective droplets; and (e) recovering the individual enzyme particles from the second liquid phase. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification