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Stabilization of enzymes in an emulsion by cross-linking

  • US 7,700,335 B2
  • Filed: 01/27/2005
  • Issued: 04/20/2010
  • Est. Priority Date: 01/28/2004
  • Status: Expired due to Fees
First Claim
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1. A process for producing enzyme particles comprising:

  • (a) providing an emulsion of droplets of a first liquid phase dispersed in a second liquid phase, with the one liquid phase being a hydrophilic phase and the other liquid phase being a hydrophobic phase which is immiscible with the hydrophilic phase, and with enzyme molecules being located at or within interfacial boundaries of the droplets and the second liquid phase;

    (b) adding a temporary protectant to the hydrophilic phase and/or to the hydrophobic phase and/or to the emulsion, wherein the temporary protectant occupies active sites of the enzyme, thereby inhibiting occupation of the active sites by a cross-linking agent or reaction of a cross-linking agent with the active sites;

    (c) adding a cross-linking agent to the hydrophilic phase and/or to the hydrophobic phase and/or to the emulsion;

    (d) cross-linking, by means of the cross-linking agent, the enzyme molecules of the respective droplets; and

    (e) recovering the individual enzyme particles from the second liquid phase.

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