Nucleic acid molecules encoding transmembrane serine proteases, the encoded proteins and methods based thereon
First Claim
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1. An isolated, substantially purified single-chain polypeptide, consisting only of a protease domain of a type-II membrane-type serine protease (MTSP) or a proteolytically active fragment thereof as a single chain, wherein:
- a free Cys, which is in the protease domain and which is normally disulfide bonded to a Cys in the pro domain of the full length MTSP, is replaced with another amino acid; and
the MTSP protease domain or the proteolytically active fragment thereof has serine protease activity as a single chain.
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Abstract
Provided herein is are polypeptides that include the protease domain of a type II transmembrane serine protease (MTSP) as a single chain. Methods using the polypeptides to identify compounds that modulate the protease activity of an MTSP are provided. Also provided are MTSPs designated MTSP3 and MTSP4 and a form of an MTSP designated MTSP6.
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Citations
38 Claims
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1. An isolated, substantially purified single-chain polypeptide, consisting only of a protease domain of a type-II membrane-type serine protease (MTSP) or a proteolytically active fragment thereof as a single chain, wherein:
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a free Cys, which is in the protease domain and which is normally disulfide bonded to a Cys in the pro domain of the full length MTSP, is replaced with another amino acid; and the MTSP protease domain or the proteolytically active fragment thereof has serine protease activity as a single chain. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
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Specification