Method for determining an analyte
First Claim
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1. A method for determining an analyte in a sample, the method comprising:
- contacting a sample that might contain the analyte being determined with a first surface so that the analyte possibly contained in the sample is bound to the first surface;
bringing a second surface to which a probe that can bind the analyte is bound close to the first surface-so that a probe comprising a marker and the analyte can interact, the binding of the probe to the analyte and the binding of the analyte to the first surface being more stable under an externally applied tensile force than the binding of the probe to the second surface;
separating the second surface and the first surface from each other; and
determining whether the probe comprising a marker is bound to the first surface and/or how much probe is bound to the second surface.
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Abstract
The present invention relates to a method for determining an analyte in a sample. According to the method of the present invention, a probe is transferred from a stamp member onto an analyte that is bound to a support member. The present invention is particularly suitable for microarrays.
41 Citations
21 Claims
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1. A method for determining an analyte in a sample, the method comprising:
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contacting a sample that might contain the analyte being determined with a first surface so that the analyte possibly contained in the sample is bound to the first surface; bringing a second surface to which a probe that can bind the analyte is bound close to the first surface-so that a probe comprising a marker and the analyte can interact, the binding of the probe to the analyte and the binding of the analyte to the first surface being more stable under an externally applied tensile force than the binding of the probe to the second surface; separating the second surface and the first surface from each other; and determining whether the probe comprising a marker is bound to the first surface and/or how much probe is bound to the second surface. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21)
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Specification