Information acquisition method, information acquisition apparatus and disease diagnosis method

• US 7,701,138 B2
• Filed: 12/28/2005
• Issued: 04/20/2010
• Est. Priority Date: 07/02/2003
• Status: Expired due to Fees

• Current Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Original Assignee: Canon Kabushiki Kaisha (Canon Inc.)
• Inventors: Kobayashi, Shin, Nakamura, Kumi, Ban, Kazuhiro, Kuge, Katsuaki, Hashimoto, Hiroyuki, Okamoto, Tadashi, Komatsu, Manabu, Imamura, Takeshi
• Primary Examiner(s): Carlson; Karen Cochrane
• Assistant Examiner(s): Shen; Bin
• Application Number:
  US11/319,233
• Publication Number:
  US 20060183235A1
• Time in Patent Office: 1,574 Days
• Field of Search: None
• US Class Current: 313/564
• CPC Class Codes: G01N 33/4833 of solid biological material, e.g. tissue samples, cell cultures tissue in vivo A61B5/00; cell suspensions G01N33/48735
• CPC Class Codes: G01N 33/6848 Methods of protein analysis involving mass spectrometry

Abstract

An information acquisition method for acquiring information on a target object, that includes a step of promoting ionization of the target object using a substance for promoting ionization of the target object to cause the target object to emit, and a step of acquiring information on the mass of the flew target object using time-of-flight secondary ion mass spectrometry.

Claims

1. A method for acquiring information on a target object, comprising the steps of:

applying a solution containing an Ag ion or an Na ion to the target object;

irradiating the target object with an ion beam to ionize the target object after applying the solution; and

acquiring information on the mass of the target object that ionizes using time-of-flight secondary ion mass spectrometry after irradiating the target object, wherein the target object that ionizes is either of;

(1) an ion corresponding to a mass obtained by addition to a parent molecule of the target object of an Ag ion or an Na ion;

or

(2) an ion corresponding to a mass obtained by addition to a partial structure of the target object of an Ag ion or an Na ion;

wherein the target object is any of;

a protein;

a peptide;

or a metabolite.

2. The method according to claim 1, wherein information on a state of two-dimensional distribution of the target object obtained by scanning of a primary ion is acquired based on a result of detection of the target object that ionizes.

3. The method according to claim 1, wherein the applying step is performed utilizing a silver mirror reaction.

4. A method for acquiring information on a target object, comprising the steps of:

applying a solution containing the target object and an Ag ion or an Na ion to a substrate;

irradiating the target object with an ion beam to ionize the target object after applying the solution; and

acquiring information on the mass of the target object that ionizes using time-of-flight secondary ion mass spectrometry after irradiating the target object, wherein the target object that ionizes is either of;

(1) an ion corresponding to a mass obtained by addition to a parent molecule of the target object of an Ag ion or an Na ion;

or

(2) an ion corresponding to a mass obtained by addition to a partial structure of the target object of an Ag ion or an Na ion;

wherein the target object is any of;

a protein;

a peptide;

or a metabolite.

5. The method according to claim 4, wherein information on a state of two-dimensional distribution of the target object obtained by scanning of a primary ion is acquired based on a result of detection of the target object that ionizes.