Digital karyotyping

• US 7,704,687 B2
• Filed: 11/13/2003
• Issued: 04/27/2010
• Est. Priority Date: 11/15/2002
• Status: Active Grant

• Current Assignee: Johns Hopkins University
• Original Assignee: Johns Hopkins University
• Inventors: Wang, Tian-Li, Kinzler, Kenneth, Velculescu, Victor, Vogelstein, Bert
• Primary Examiner(s): Vogel; Nancy
• Assistant Examiner(s): HIBBERT, CATHERINE S
• Application Number:
  US10/705,874
• Publication Number:
  US 20040096892A1
• Time in Patent Office: 2,357 Days
• Field of Search: None
• US Class Current: 435/6.13
• CPC Class Codes: C12Q 1/6841 In situ hybridisation
• CPC Class Codes: C12Q 2521/313 Type II endonucleases, i.e. cutting outside recognition site
• CPC Class Codes: C12Q 2537/157 A reaction step characterised by the number of molecules incorporated or released
• CPC Class Codes: C12Q 2565/102 Multiple non-interacting labels

Abstract

Alterations in the genetic content of a cell underlie many human diseases, including cancers. A method called Digital Karyotyping provides quantitative analysis of DNA copy number at high resolution. This approach involves the isolation and enumeration of short sequence tags from specific genomic loci. Analysis of human cancer cells using this method identified gross chromosomal changes as well as amplifications and deletions, including regions not previously known to be altered. Foreign DNA sequences not present in the normal human genome could also be readily identified. Digital Karyotyping provides a broadly applicable means for systematic detection of DNA copy number changes on a genomic scale.

Claims

1. A method of karyotyping a genome of a test eukaryotic cell, comprising:

sequencing a population of pieces of the genome of the test eukaryotic cell to provide nucleotide sequence of said pieces;

matching, in silico, pieces of the genome to genomic locations using the nucleotide sequence of said pieces;

counting the pieces within windows of a selected size throughout the genome to determine number of pieces as a function of genomic location, wherein each window comprises a plurality of genomically clustered pieces;

comparing the number of pieces enumerated within each window for the test eukaryotic cell to the average number of pieces in windows of the selected size throughout the genome to obtain piece densities per window, wherein the piece densities per window represent the karyotype of the genome of the test eukaryotic cell.

2. The method of claim 1 wherein the pieces within each of the windows map within 40 kb.

3. The method of claim 1 wherein the pieces within each of the windows map within 200 kb.

4. The method of claim 1 wherein the pieces within each of the windows map within 600 kb.

5. The method of claim 1 wherein the pieces within each of the windows map within 4 Mb.

6. The method of claim 1 wherein the pieces are defined by the presence of a BcgI restriction endonuclease recognition site which is flanked by 12 nucleotides on either end.

7. The method of claim 1 further comprising the steps of:

comparing piece densities per window for the test eukaryotic cell to piece densities of a reference eukaryotic cell; and

identifying aneuploidy if (a) pieces of one or more autosomes are determined to be present in the test eukaryotic cell relative to the reference eukaryotic cell at a ratio of 1.5 or greater or less than 0.7;

or (b) pieces of one or more sex chromosomes in a male are determined to be present in the test eukaryotic cell relative to a reference male eukaryotic cell at a ratio of 1.5 or greater or less than 0.7;

or (c) pieces of X chromosomes in a female are determined to be present in the test eukaryotic cell relative to a reference male eukaryotic cell at a ratio of 3 or greater or less than 1.5 or relative to a reference female eukaryotic cell at a ratio of 1.5 or greater or less than 0.7.

8. The method of claim 1 wherein pieces representing less than 15% of the genome of the eukaryotic cell are sequenced, matched, and counted.

21. The method of claim 1 or 9 wherein the sequencing is performed by automated nucleotide sequence determination.

22. The method of claim 1 or 9 wherein between 100,000 and 1,000,000 pieces are sequenced and matched.

23. The method of claim 1 or 9 further comprising the step of:

comparing piece densities per window for the test eukaryotic cell to piece densities of a reference eukaryotic cell.

24. The method of claim 1 or 9 wherein the selected size is less than or equal to 40 kb.

25. The method of claim 1 or 9 wherein the selected size is less than or equal to 200 kb.

26. The method of claim 1 or 9 wherein the selected size is less than or equal to 600 kb.

27. The method of claim 1 or 9 wherein the selected size is less than or equal to 4 Mb.

9. A method of determining differences in copy number of portions of the genome of a test eukaryotic cell, comprising:

sequencing a population of pieces of the genome of the test eukaryotic cell to provide nucleotide sequence of said pieces;

matching, in silico, pieces of the genome to genomic locations using the nucleotide sequence of said pieces;

dynamically counting the pieces within a moving window of a selected size to determine number of pieces as a function of genomic location, wherein the window comprises a plurality of genomically clustered pieces;

comparing number of pieces enumerated within the window at a genomic location to an average number of pieces in windows of the selected size throughout the genome to obtain piece density per window, wherein a difference in piece density per window between windows reflects a difference in copy number between portions of the genome.

10. The method of claim 9 wherein the difference in copy number is due to gain or loss of a whole chromosome.

11. The method of claim 9 wherein the difference in copy number is due to a gain or loss of a chromosomal arm.

12. The method of claim 9 wherein the difference in copy number is due to an interstitial amplification.

13. The method of claim 9 wherein the difference in copy number is due to an interstitial deletion.

14. The method of claim 9 wherein the pieces within the window map within 40 kb.

15. The method of claim 9 wherein the pieces within the window map within 200 kb.

16. The method of claim 9 wherein the pieces within the window map within 600 kb.

17. The method of claim 9 wherein the pieces within the window map within 4 Mb.

18. The method of claim 9 wherein the pieces are defined by the presence of a BcgI restriction endonuclease recognition site which is flanked by 12 nucleotides on either end.

19. The method of claim 9 further comprising the steps of:

comparing piece densities per window for the test eukaryotic cell to piece densities of a reference eukaryotic cell; and

identifying aneuploidy if (a) pieces of one or more autosomes are determined to be present in the test eukaryotic cell relative to the reference eukaryotic cell at a ratio of 1.5 or greater or less than 0.7;

or (b) pieces of one or more sex chromosomes in a male are determined to be present in the test eukaryotic cell relative to a reference male eukaryotic cell at a ratio of 1.5 or greater or less than 0.7;

or (c) pieces of X chromosomes in a female are determined to be present in the test eukaryotic cell relative to a reference male eukaryotic cell at a ratio of 3 or greater or less than 1.5 or relative to a reference female eukaryotic cell at a ratio of 1.5 or greater or less than 0.7.

20. The method of claim 9 wherein pieces representing less than 15% of the genome of the eukaryotic cell are sequenced, matched, and dynamically counted.