Active programmable matrix devices

• US 7,704,726 B2
• Filed: 08/17/2006
• Issued: 04/27/2010
• Est. Priority Date: 11/01/1993
• Status: Expired due to Fees

• Current Assignee: Gamida for Life B.V.
• Original Assignee: Gamida for Life B.V.
• Inventors: Sosnowski, Ronald George, Juncosa, Robert David, Jackson, Thomas Ratcliffe, Heller, Michael James, O'Connell, James Patrick
• Primary Examiner(s): FORMAN, BETTY J
• Application Number:
  US11/505,679
• Publication Number:
  US 20070054299A1
• Time in Patent Office: 1,349 Days
• Field of Search: None
• US Class Current: 435/283.1
• CPC Class Codes: B01J 2219/00317 Microwell devices, i.e. having large numbers of wells
• CPC Class Codes: B01J 2219/00418 using pressure
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/00621 by physical means, e.g. trenches, raised areas
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00644 the porous medium being present in discrete locations, e.g. gel pads
• CPC Class Codes: B01J 2219/00653 the compounds being bound to electrodes embedded in or on the solid supports
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01L 2200/0647 Handling flowable solids, e.g. microscopic beads, cells, particles
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2200/12 Specific details about manufacturing devices
• CPC Class Codes: B01L 2300/0636 Integrated biosensor, microarrays
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2300/0877 Flow chambers
• CPC Class Codes: B01L 2400/0421 electrophoretic flow
• CPC Class Codes: B01L 3/5027 by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip B01L3/5023 takes precedence; micromixers B01F33/30; microreactors for synthesis B01J19/0093; microcapillary devices in general B81B1/00
• CPC Class Codes: B01L 3/502707 characterised by the manufacture of the container or its components by shaping or joining plastic parts B29C59/00, B29C65/00; by laminating B32B37/00; manufacture of microstructural devices in general B81C
• CPC Class Codes: C07H 21/00 Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
• CPC Class Codes: G01N 2035/00158 Elements containing microarrays, i.e. "biochip"

Abstract

A system for performing molecular biological diagnosis, analysis and multistep and multiplex reactions utilizes a selfaddressable, selfassembling microelectronic system for actively carrying out controlled reactions in microscopic formats. The device includes a power supply and waveform generator adapted to supply a DC bias and superimposed AC signal to the system through an interface to the array of microlocations.

Claims

1. An active biological device having an array of microlocations, including at least a first microlocation and a second microlocation, the device adapted to receive a solution including one or more charged entities, the device comprising:

a controller, wherein the controller is a computer;

a power supply and waveform generator, the power supply and waveform generator adapted to output a DC bias and superimposed AC signal at an output under control of the controller; and

an interface unit coupled to the output of the power supply and waveform generator, wherein the interface includes a plurality of relays which couple the output of the power supply and waveform generator selectively to the microlocations of the array of microlocations, wherein the controller provides control signals to the interface so that the relays are under control of the controller, and wherein the controller directs the relays to select the polarity of the voltages applied to the microlocations, to permit selection or nonselection of a path from the power supply and waveform generator to the microlocations, and to select an amount of potential or current provided to the microlocations.

2. The device of claim 1 wherein the DC bias is a current bias.

3. The device of claim 1 wherein the DC bias is a voltage bias.

4. The device of claim 1, wherein the AC signal is non-repetitive.

5. The device of claim 1 wherein the AC signal is zero at time zero.