Differential labelling method

• US 7,704,755 B2
• Filed: 05/28/2002
• Issued: 04/27/2010
• Est. Priority Date: 05/28/2001
• Status: Active Grant

• Current Assignee: Leica Biosystems Newcastle Ltd. (Danaher Corporation)
• Original Assignee: Kreatech Biotechnology BV (Danaher Corporation)
• Inventors: Talman, Eduard Gerhard, Heetebrij, Robert Jochem, Veuskens, Jacky Theo Maria, van Gijlswijk, Robertus Petrus Maria
• Primary Examiner(s): Jung; Unsu
• Assistant Examiner(s): LUM, LEON YUN BON
• Application Number:
  US10/156,730
• Publication Number:
  US 20030060647A1
• Time in Patent Office: 2,891 Days
• Field of Search: 435/4, 435/6, 435/7.1, 435/7.5, 436/518, 436/524, 436/525, 436/56, 436/106, 436/119, 436/163, 556/16
• US Class Current: 436/525
• CPC Class Codes: G01N 33/58 involving labelled substances G01N33/53 takes precedence
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH

Abstract

The invention relates to a method for differentially labelling one or more entities, together comprising distinct reactive sites. The invention further relates to an entity that has been labelled by a method according to the invention and to a diagnostic kit comprising a labelled entity and to a diagnostic kit to employ a method according to the invention.

Claims

1. A method for differentially forming a complex of a platinum compound and an entity or at least one entity of a mixture of two or more entities, the method comprising:

forming a complex of a platinum compound and an entity, said entity comprising one or more sulphur-containing reactive sites and one or more nitrogen-containing reactive sites, wherein said platinum compound is reacted with said entity such that substantially only nitrogen containing reactive sites are linked to said platinum compound;

or

forming a complex of a platinum compound and at least one entity of a mixture of two or more entities, said at least one entity or said entities together comprising one or more sulphur-containing reactive sites and one or more nitrogen-containing reactive sites, wherein said platinum compound is reacted with said at least one entity or said entities together, such that substantially only nitrogen containing reactive sites are linked to said platinum compound,

wherein said sulphur containing site or sites are first differentially shielded by a shielding moiety and thereafter said platinum compound is differentially linked to the one or more nitrogen containing reactive sites;

wherein said entity or said entities are selected from the group consisting of amino acids, peptides, oligopeptides, polypeptides, proteins, immunoglobulins, enzymes, synzymes, phospholipids, glycoproteins, nucleic acids, nucleosides, nucleotides, oligonucleotides, polynucleotides, peptide nucleic acids, peptide nucleic acid oligomers, peptide nucleic acid polymers, amines and aminoglycosides; and

wherein said shielding moiety is a trans-platinum compound.

2. The method according to claim 1, wherein the shielding moiety is selectively removed from the shielded reactive site, after the platinum compound has been reacted such that said platinum is differentially linked to said entity.

3. The method according to claim 1, wherein said method comprises:

differentially shielding, by means of a shielding moiety, sulfur-containing reactive site or sites of at least one entity of a mixture of two or more entities, said entity or entities together comprising one or more sulphur-containing reactive sites and one or more nitrogen-containing reactive sites;

forming a complex of a platinum compound and at least one entity of a mixture of two or more entities, said at least one entity or said entities together, comprising one or more shielded sulphur-containing reactive sites and one or more nitrogen-containing reactive sites;

wherein said platinum compound is reacted with said at least one entity such that substantially only nitrogen-containing reactive sites are linked to said platinum compound.