Method and system for solubilizing protein
First Claim
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1. A recirculating method of solubilizing protein and destroying prions comprising the following steps in the following order:
- first, applying an alkali to an untreated protein source to form a slurry, wherein the alkali is added in an amount sufficient to induce hydrolysis of the protein source when the slurry is heated;
second, heating the slurry to a temperature sufficient to allow hydrolysis of protein in the protein source to obtain a reaction liquid comprising small peptides and free amino acids, and further comprising solid residues;
third, holding the reaction liquid at an elevated temperature between 125-250°
C. for a time period sufficient to destroy prions in the reaction liquid;
fourth, separating the solid residues from the reaction liquid;
fifth, neutralizing the reaction liquid with acid or an acid source to produce a neutralized liquid comprising small peptides and free amino acids;
sixth, concentrating the neutralized liquid to produce concentrated liquid comprising small peptides and free amino acids and water; and
seventh, returning the water to the slurry before or during the heating step.
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Abstract
A process for solubilization of protein including application of an alkali, such as lime, and heating. The process may also involve lime recovery and may be accomplished in a single stage or two stages to separate protein solubilized from labile and recalcitrant sources. Systems and devices for use in such process, including a continuous stirred tank reactor and a plug flow reactor are also involved.
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Citations
31 Claims
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1. A recirculating method of solubilizing protein and destroying prions comprising the following steps in the following order:
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first, applying an alkali to an untreated protein source to form a slurry, wherein the alkali is added in an amount sufficient to induce hydrolysis of the protein source when the slurry is heated; second, heating the slurry to a temperature sufficient to allow hydrolysis of protein in the protein source to obtain a reaction liquid comprising small peptides and free amino acids, and further comprising solid residues; third, holding the reaction liquid at an elevated temperature between 125-250°
C. for a time period sufficient to destroy prions in the reaction liquid;fourth, separating the solid residues from the reaction liquid; fifth, neutralizing the reaction liquid with acid or an acid source to produce a neutralized liquid comprising small peptides and free amino acids; sixth, concentrating the neutralized liquid to produce concentrated liquid comprising small peptides and free amino acids and water; and seventh, returning the water to the slurry before or during the heating step. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15)
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16. The recirculating method of solubilizing protein comprising the following steps in the following order:
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first, applying an alkali to a protein source to form a slurry, wherein the alkali is added in an amount sufficient to induce hydrolysis of the protein source when the slurry is heated; second, heating the slurry to a temperature sufficient to allow hydrolysis of protein in the protein source to obtain a reaction liquid comprising solid residues; third, separating the solid residues from the reaction liquid; fourth, neutralizing the reaction liquid with acid or an acid source to produce a neutralized liquid; fifth, concentrating the neutralized liquid to produce concentrated liquid and water; and sixth, returning the water to the slurry before or during the heating step. - View Dependent Claims (17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31)
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Specification