Polynucleotides encoding anti-ganglioside antibodies

• US 7,709,615 B2
• Filed: 07/15/2004
• Issued: 05/04/2010
• Est. Priority Date: 07/15/2003
• Status: Active Grant

• Current Assignee: Chugai Seiyaku Kabushiki Kaisha (Roche Holding AG)
• Original Assignee: Chugai Seiyaku Kabushiki Kaisha (Roche Holding AG)
• Inventors: Irie, Reiko, Tsunoda, Hiroyuki, Tsuchiya, Masayuki, Igawa, Tomoyuki, Sekimori, Yasuo
• Primary Examiner(s): OUSPENSKI, ILIA I
• Application Number:
  US10/564,665
• Publication Number:
  US 20070154469A1
• Time in Patent Office: 2,119 Days
• Field of Search: None
• US Class Current: 536/23.1
• CPC Class Codes: C07K 16/00 Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies antibodies with enzymatic activity, e.g. abzymes C12N9/0002
• CPC Class Codes: C07K 16/3084 against tumour-associated gangliosides
• CPC Class Codes: C07K 2317/21 from primates, e.g. man
• CPC Class Codes: C07K 2317/52 Constant or Fc region; Isotype
• CPC Class Codes: C07K 2317/734 Complement-dependent cytotoxicity [CDC]
• CPC Class Codes: G01N 33/6854 Immunoglobulins
• CPC Class Codes: G01N 33/686 Anti-idiotype

Abstract

IgM can be obtained in the form of a pentamer by placing the genes encoding the H, L, and J chains on the same vector to transform appropriate host cells. The gene encoding the J chain may be introduced by co-transfection. When no J chain is expressed, the IgM is produced as a hexamer. The transformants obtained according to the present invention achieve a high yield of IgM. The present invention also provides methods which enable separation and quantification of polymeric IgM.

Claims

1. An isolated polynucleotide comprising:

a) the nucleotide sequence of SEQ ID NO;

1 or a nucleotide sequence encoding the amino acid sequence of SEQ ID NO;

2;

b) the nucleotide sequence of SEQ ID NO;

3 or a nucleotide sequence encoding the amino acid sequence of SEQ ID NO;

4;

c) the nucleotide sequence of SEQ ID NO;

19 or a nucleotide sequence encoding the amino acid sequence of SEQ ID NO;

20;

or

d) the nucleotide sequence of SEQ ID NO;

21 or a nucleotide sequence encoding the amino acid sequence of SEQ ID NO;

22.

2. The isolated polynucleotide of claim 1, wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO:

1 or a nucleotide sequence encoding the amino acid sequence of SEQ ID NO;

2.

3. The isolated polynucleotide of claim 2, wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO:

1.

4. The isolated polynucleotide of claim 2, wherein the polynucleotide comprises a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:

2.

5. An expression vector comprising the polynucleotide of claim 2.

6. A transformed cell comprising the expression vector of claim 5.

7. A method of producing a polypeptide comprising the amino acid sequence of SEQ ID NO:

2, the method comprising culturing the transformed cell of claim 6 and collecting the polypeptide.

8. The isolated polynucleotide of claim 1, wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO:

3 or a nucleotide sequence encoding the amino acid sequence of SEQ ID NO;

4.

9. The isolated polynucleotide of claim 8, wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO:

3.

10. The isolated polynucleotide of claim 8, wherein the polynucleotide comprises a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:

4.

11. An expression vector comprising the polynucleotide of claim 8.

12. A transformed cell comprising the expression vector of claim 11.

13. A method of producing a polypeptide comprising the amino acid sequence of SEQ ID NO:

4, the method comprising culturing the transformed cell of claim 12 and collecting the polypeptide.

14. The isolated polynucleotide of claim 1, wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO:

19 or a nucleotide sequence encoding the amino acid sequence of SEQ ID NO;

20.

15. The isolated polynucleotide of claim 14, wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO:

19.

16. The isolated polynucleotide of claim 14, wherein the polynucleotide comprises a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:

20.

17. An expression vector comprising the polynucleotide of claim 14.

18. A transformed cell comprising the expression vector of claim 17.

19. A method of producing a polypeptide comprising the amino acid sequence of SEQ ID NO:

20, the method comprising culturing the transformed cell of claim 18 and collecting the polypeptide.

20. The isolated polynucleotide of claim 1, wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO:

21 or a nucleotide sequence encoding the amino acid sequence of SEQ ID NO;

22.

21. The isolated polynucleotide of claim 20, wherein the polynucleotide comprises the nucleotide sequence of SEQ ID NO:

21.

22. The isolated polynucleotide of claim 20, wherein the polynucleotide comprises a nucleotide sequence encoding the amino acid sequence of SEQ ID NO:

22.

23. An expression vector comprising the polynucleotide of claim 20.

24. A transformed cell comprising the expression vector of claim 23.

25. A method of producing a polypeptide comprising the amino acid sequence of SEQ ID NO:

22, the method comprising culturing the transformed cell of claim 24 and collecting the polypeptide.