Non-invasive determination of blood components

• US 7,711,403 B2
• Filed: 04/04/2002
• Issued: 05/04/2010
• Est. Priority Date: 04/05/2001
• Status: Active Grant

• Current Assignee: Rhode Island Hospital
• Original Assignee: Rhode Island Hospital
• Inventors: Ernsting, Kevin S., Suner, Selim, Jay, Gregory D.
• Primary Examiner(s): Casler; Brian
• Assistant Examiner(s): Roy; Baisakhi
• Application Number:
  US10/116,258
• Publication Number:
  US 20030002722A1
• Time in Patent Office: 2,952 Days
• Field of Search: 600/407, 600/473, 600/474, 600/476, 600/477, 600/558, 600/310, 600/316, 600/322, 600/368, 356/456
• US Class Current: 600/407
• CPC Class Codes: A61B 2560/0233 Optical standards
• CPC Class Codes: A61B 3/14 Arrangements specially adapted for eye photography apparatus or arrangements for taking photographs per se G03B
• CPC Class Codes: A61B 5/0059 using light, e.g. diagnosis by transillumination, diascopy, fluorescence A61B5/0093, A61B5/1455, A61B5/02416 and A61B5/0261 take precedence
• CPC Class Codes: A61B 5/1034 by means of colour cards
• CPC Class Codes: A61B 5/14546 for measuring analytes not otherwise provided for, e.g. ions, cytochromes
• CPC Class Codes: A61B 5/1455 using optical sensors, e.g. spectral photometrical oximeters
• CPC Class Codes: A61B 5/443 Evaluating skin constituents, e.g. elastin, melanin, water

Abstract

A method of estimating an amount of a substance in a bodily fluid from the color of a tissue surface of a subject includes capturing an image including at least a portion-of-interest of the subject and at least a reference portion of a color reference, the image being a digital image of pixels of at least one color, the image including a component value for each pixel for each of the at least one color, obtaining a first value associated with at least one component value corresponding to the portion-of-interest of the subject, obtaining a second value associated with at least one component value corresponding to the reference portion, and calculating an estimated amount of the substance using the first and second values.

Claims

1. A non-invasive method of estimating an amount of hemoglobin in a bodily fluid from the color of a perfused tissue surface of a subject using a digital camera, the method comprising:

non-invasively capturing, using the digital camera, a single image of light reflected from the perfused tissue surface of the subject and at least a reference portion of a known color reference simultaneously, the single image being a single image of pixels of a plurality of colors, the single image including red, green, and blue component values for each pixel wherein the perfused tissue surface of the subject is a conjunctiva of an eyelid;

obtaining from the single image, using at least one processor in communication with the digital camera, red, green, and blue tissue values associated with the red, green, and blue component values, respectively, corresponding to a portion-of-interest of the single image of the perfused tissue surface of the subject;

obtaining from the single image, using the at least one processor in communication with the digital camera, red, green, and blue reference values associated with the red, green, and blue component values, respectively, corresponding to the reference portion; and

calculating, using the at least one processor in communication with the digital camera, an estimated hemoglobin concentration amount as a function of the red, green, and blue tissue values and the red, green, and blue reference values.

2. The method of claim 1 wherein obtaining the red, green, and blue tissue values and the red, green, and blue reference values comprises averaging component values in a first portion of the single image of the perfuse tissue surface and a second portion of the reference portion, respectively, for each of the red, green, and blue component values.

3. The method of claim 2 wherein the calculating comprises using first and second empirically-predetermined weighting constants associated with the red, green, and blue tissue values and the red, green, and blue reference values respectively.

4. The method of claim 1 further comprising selecting, using the at least one processor in communication with the digital camera, a first portion of the single image of the perfused tissue surface and a second portion of the color reference, wherein the red, green, blue tissue values are obtained from the first portion, and wherein the red, green, and blue reference values are obtained from the second portion.

5. The method of claim 1 wherein the capturing, the obtaining of red, green, and blue tissue values, the obtaining of the red, green, and blue reference values, and the calculating are performed in real time.

6. The method of claim 1 further comprising displaying, on a display in communication with the at least one processor, the estimated hemoglobin concentration amount.

7. The method of claim 6 wherein the estimated hemoglobin concentration amount is displayed in units of grams per deciliter (g/dl).

8. The method of claim 1 wherein capturing the single image includes capturing an image of the known color reference adjacent to the perfused tissue surface of the subject.

9. The method of claim 1 wherein capturing the single image includes capturing an image of a known color reference that is gray.

10. The method of claim 1 further comprising everting a lower eyelid of the subject to expose the conjunctiva of the eyelid.

11. A non-invasive method of determining factors that influence estimation of an amount of hemoglobin in a bodily fluid from the color of a perfused tissue surface of a subject, the method comprising:

non-invasively capturing, using a digital camera, a single image of light reflected from the perfused tissue surface of the subject and at least a reference portion of a known color reference simultaneously, the single image including pixels of a plurality of colors, the single image including red, green, and blue component values for each pixel, wherein the perfused tissue surface of the subject is a conjunctiva of an eyelid;

establishing, using at least one processor in communication with the digital camera, red, green, and blue tissue factors associated with a perfused tissue surface portion-of-interest of the single image of the subject, and red, green, and blue reference factors associated with the digital camera and a reference portion-of-interest of the single image of the subject;

calculating, using the at least one processor in communication with the digital camera, an estimated hemoglobin concentration amount using the single image and as a function of the red, green, and blue component values and the red, green and blue tissue factors, and the red, green, and blue reference factors;

comparing, using the at least one processor in communication with the digital camera, the estimated hemoglobin concentration amount with a corresponding known amount of hemoglobin in the subject; and

adjusting, if the estimated hemoglobin concentration amount has other than a desired relationship with the known amount, at least one of the red, green, and blue tissue factors, and the red, green, and blue reference factors using the at least one processor in communication with the digital camera.

12. The method of claim 11 further comprising repeating the calculating, the comparing, and the adjusting until the estimated amount of hemoglobin has the desired relationship with the known amount of hemoglobin.

13. The method of claim 12 further comprising storing, in a memory in communication with the at least one processor, the red, green, and blue tissue factors and the red, green, and blue reference factors when the estimated amount of hemoglobin has the desired relationship with the known amount of hemoglobin.

14. The method of claim 11 further comprising selecting a first region of the single image of the perfused tissue surface and a second region of the reference color using the at least one processor in communication with the digital camera.

15. The method of claim 14 wherein the calculating uses averages of the red, green and blue component values for each color over the first and second regions, respectively.

16. The method of claim 15 wherein the calculating uses ratios of the averages of the red, green, and blue component values for each color over the first and second regions, respectively.

17. The method of claim 11 further comprising everting a lower eyelid of the subject to expose the conjunctiva of the eyelid.

18. A system for determining a level of hemoglobin in a bodily fluid from the color of a perfused tissue surface of a subject, the system comprising:

an imaging module configured to non-invasively capture a single color image of the perfused tissue surface of the subject and a known color reference object, wherein the perfused tissue surface is a conjunctiva of an eyelid;

a color separator module in communication with the imaging module and configured to decompose the single color image into sub-images of component colors, the sub-images comprising digital component values corresponding to pixels of the single image;

a portion selector module in communication with the color separator module and configured to select a first window of the single image of the perfused tissue surface and to select a second window of the single image of the color reference object; and

a hemoglobin estimator module in communication with the portion selector and configured to calculate an estimated hemoglobin concentration level as a function of window values associated with component values corresponding to the first and second windows.

19. The system of claim 18 wherein the portion selector module is further configured to average the component values of each sub-image to produce the window values, with one window value for each sub image for each of the first and second windows.

20. The system of claim 18 wherein the color separator module, the portion selector module, and the hemoglobin estimator module each comprise computer-executable instructions, stored on a computer-readable medium, for causing a computer to perform actions as recited in claim 18.

21. The system of claim 18 further comprising a display coupled to the hemoglobin estimator module and configured to display indicia of the estimated hemoglobin level.

22. A non-invasive method of estimating a concentration of hemoglobin in blood of a subject from the color of a perfused tissue surface of the subject using a digital camera, the method comprising:

non-invasively capturing, using the digital camera, a single image of light reflected from the perfused tissue surface of the subject and at least a reference portion of a known color reference simultaneously, the single image being a single image of pixels a plurality of colors, the single image including red, green and blue component values for each pixel for each of the at least one color, wherein the perfused tissue surface of the subject is a conjunctiva of an eyelid;

obtaining from the single image, using at least one processor in communication with the digital camera, red, green, and blue tissue values associated with the red, green, and blue component values, respectively, corresponding to a portion-of-interest of the single image of the perfused tissue surface of the subject;

obtaining, from the single image, using the at least one processor in communication with the digital camera, red, green, and blue reference values associated with the red, green, and blue component values, respectively, corresponding to the reference portion; and

calculating, using the at least one processor in communication with the digital camera, an estimated hemoglobin value indicative of a concentration of hemoglobin in the blood of the subject as a function of the red, green, and blue tissue values and the red, green, and blue reference values.

23. The method of claim 22 further comprising everting a lower eyelid of the subject to expose the conjunctiva of the eyelid.

24. A non-invasive method of determining factors that influence estimation of a concentration of hemoglobin in blood of a subject from the color of a perfused tissue surface of the subject, the method comprising:

non-invasively capturing, using a digital camera, a single image of light reflected from the perfused tissue surface of the subject and at least a reference portion of a known color reference simultaneously, the single image including pixels of a plurality of colors, the single image including red, green and blue component values for each pixel, wherein the perfused tissue surface of the subject is a conjunctiva of an eyelid;

establishing, using at least one processor in communication wiht the digital camera, red, green, and blue tissue factors associated with the digital camera and a tissue portion-of-interest of the single image of the subject, and red, green, and blue reference factors associated with the digital camera and a reference portion-of-interest of the single image of the subject;

calculating, using the at least one processor in communication with the digital camera, an estimated hemoglobin level, using the single image, that is indicative of a concentration of hemoglobin in the blood of the subject as a function of the red, green, and blue component values and the red, green, and blue tissue factors and the red, green, and blue reference factors;

comparing, using the at least one processor in communication with the digital camera, the estimated hemoglobin level with a corresponding known amount of hemoglobin in the subject; and

adjusting, using the at least one processor in communication with the digital camera, if the estimated hemoglobin level has other than a desired relationship with the known amount, at least one of the red, green, and blue tissue factors and the red, green, and blue reference factors, respectively.

25. The method of claim 24 further comprising everting a lower eyelid of the subject to expose the conjunctiva of the eyelid.