Biosensor

• US 7,713,406 B2
• Filed: 05/06/2005
• Issued: 05/11/2010
• Est. Priority Date: 09/12/1997
• Status: Expired due to Fees

• Current Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories)
• Original Assignee: Abbott Diabetes Care Incorporated (Abbott Laboratories)
• Inventors: Mure, Hiroki, Shirakawa, Hiroshi, Gotoh, Masao
• Primary Examiner(s): NOGUEROLA, ALEXANDER STEPHAN
• Application Number:
  US11/123,230
• Publication Number:
  US 20050258052A1
• Time in Patent Office: 1,831 Days
• Field of Search: 20440301-40315, 205/777.5, 205/778, 205/792
• US Class Current: 205/792
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: G01N 27/3272 Test elements therefor, i.e. disposable laminated substrates with electrodes, reagent and channels optical biosensors G01N33/52
• CPC Class Codes: Y10S 435/817 Enzyme or microbe electrode

Abstract

A biosensor comprises a space part for sucking and housing a sample formed of two upper and lower plates, the two plates being stuck together by an adhesive layer, the space part for sucking and housing the sample being constituted so as to be partially opened in the peripheral part and partially closed by the adhesive layer, and has a working electrode having at least glucose oxidase immobilized thereon and a counter electrode on the same plane of the plate.

Claims

1. A biosensor comprising:

a proximal end and a distal end;

a first layer having a first length and a second layer having a second length, the first and second layers being fixed together by a third layer having a third length;

a tapered part located at the proximal end;

a working electrode provided on a first layer and a counter electrode provided on a second layer, wherein the tip part of the working electrode is provided on the tapered part; and

wherein leads for the working and the counter electrodes extend from the tapered part to the distal end.

2. The biosensor of claim 1, wherein the tapered part includes an opening for admitting sample.

3. The biosensor of claim 2, further comprising a space for housing a sample and the opening is in communication with the space for housing the sample.

4. The biosensor of claim 3, wherein the space has a volume that ranges from about 0.5-10 microliters.

5. The biosensor of claim 1, wherein a tip part of the counter electrode is provided on the tapered part.

6. The biosensor of claim 1, further comprising a reference electrode and a tip part of the reference electrode is provided on the tapered part.

7. The biosensor of claim 6, wherein the working electrode and reference electrode are provided on the same layer.

8. The biosensor of claim 7, wherein the same layer is the first layer and a length of the first layer is greater than a length of the second layer.

9. The biosensor of claim 6, wherein the counter electrode and reference electrode are provided on the same layer.

10. The biosensor of claim 1, wherein the third layer comprises adhesive.

11. The biosensor of claim 1, further comprising an analyte-responsive enzyme.

12. The biosensor of claim 11, wherein the analyte-responsive enzyme is selected from the group of glucose oxidase and glucose dehydrogenase.

13. The biosensor of claim 1, wherein the biosensor is connectable to a device body having a control part including a display part, and the bio sensor further comprises a biosensor insertion signal terminal for interconnecting, upon connecting the biosensor to the device body, two input terminals of the device body to start the control part of the device body.

14. A biosensor comprising:

a proximal end and a distal end,

a first plate having a first length,

a second plate having a second length,

a space formed between the two plates for housing a sample therein,

a layer having a third length for fixing the two plates together,

the space for housing the sample being partially opened at a peripheral part thereof and partially closed by the layer,

a working electrode provided on the first plate and a counter electrode provided on the second plate and fixed relative to the working electrode,

a tapered part located at the proximal end, and

a tip part of the working and counter electrodes being provided on the tapered part,

wherein leads for the working and counter electrodes extend from the tapered part to the distal end.

15. The biosensor of claim 14, further comprising an analyte-responsive enzyme.

16. The biosensor of claim 15, wherein the analyte-responsive enzyme is selected from the group of glucose oxidase and glucose dehydrogenase.

17. The biosensor of claim 14, wherein the biosensor is connectable to a device body having a control part including a display part and the biosensor further comprises a biosensor insertion signal terminal for interconnecting, upon connecting the biosensor to the device body, two input terminals of the device body to start the control part of the device body.

18. The biosensor of claim 14, wherein the tapered part includes an opening for admitting sample.

19. The biosensor of claim 18, further comprising a space for housing a sample and the opening is in communication with the space for housing a sample.

20. The biosensor of claim 19, wherein the space has a volume that ranges from about 0.5-10 microliters.

21. The biosensor of claim 14, wherein the biosensor further comprises a reference electrode and a tip part of the reference electrode is provided on the tapered part.

22. The biosensor of claim 21, wherein the working electrode and reference electrode are provided on the same layer.

23. The biosensor of claim 21, wherein the counter electrode and reference electrode are provided on the same layer.

24. A method of measuring a concentration of an analyte in a subject, the method comprising:

contacting body fluid of the subject with a biosensor to generate an electrical signal, the biosensor having a proximal end and a distal end, at least three layers, a working electrode provided on a first layer and a counter electrode provided on a second layer, a tapered part located at the proximal end, and a tip part of the working electrode provided on the tapered part, wherein the three different layers have different lengths and leads for the working and counter electrodes extend from the tapered part to the distal end, and

determining from the generated electrical signal a concentration of an analyte in body fluid.

25. The method of measuring a concentration of an analyte in the subject as claimed in claim 24, wherein the body fluid is contacted with the biosensor at the tapered part.

26. The method of measuring a concentration of an analyte in the subject as claimed in claim 25, wherein the tapered part comprises an opening in communication with a space for housing the body fluid, and the method comprises admitting the body fluid into the space through the opening in the tapered part.

27. The method of measuring a concentration of an analyte in the subject as claimed in claim 24, wherein the body fluid ranges from about 0.5 to about 10 microliters.

28. The method of measuring a concentration of an analyte in the subject as claimed in claim 24, wherein the biosensor comprises an analyte-responsive enzyme.

29. The method of measuring a concentration of an analyte in the subject as claimed in claim 28, wherein the analyte-responsive enzyme is selected from the group of glucose oxidase and glucose dehydrogenase.

30. The method of measuring a concentration of an analyte in the subject as claimed in claim 24, wherein the biosensor is connectable to a device body having a control part including a display part and the biosensor further comprises a biosensor insertion signal terminal for interconnecting, upon connecting the biosensor to the device body, two input terminals of the device body to start the control part of the device body.