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Method for in vivo delivery of active compounds using reagent conjugate

  • US 7,713,528 B1
  • Filed: 06/07/1995
  • Issued: 05/11/2010
  • Est. Priority Date: 02/18/1993
  • Status: Active Grant
First Claim
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1. A method for visualizing specific target analytes in a subject which comprises:

  • (1) providing a reagent conjugate useful for delivering active compounds to specific target analytes into a subject, said conjugate comprising(a) a first reagent comprising a specific target recognition component which comprises a Fab′

    antibody fragment which is covalently attached to a first sequence-specific component comprising a first oligonucleotide comprised of 2′

    -chlorodeoxy-guanosine which is about 20 bases long; and

    (b) a second reagent comprising an active compound component linked to a second sequence-specific component which specifically recognizes and binds to said first sequence-specific component, said second reagent-comprised of 90y bound to a 1,2-diaminocyclohexane-tetraacetate (DCTA) chelating moiety, said second sequence-specific component comprising a second oligonucleotide, said DCTA chelating moiety being covalently attached through the allylamino group at the 5-position of a cytidyl residue in said second oligonucleotide which comprises the sequence [(2′

    -chloro-deoxycytosine)3(2′

    -chloro-deoxycytosine-5-allylamine)]5, andwherein said first and second sequence-specific components (a) and (b) have been rendered nuclease resistant by the presence of one or more nucleotide analogs to said oligonucleotides;

    (2) introducing into said subject an effective amount of said reagent conjugate; and

    (3) visualizing said specific target analytes.

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