Electronic methods for the detection of analytes

• US 7,713,711 B2
• Filed: 10/30/2007
• Issued: 05/11/2010
• Est. Priority Date: 06/12/1997
• Status: Expired due to Fees

• Current Assignee: Osmetech Technology Inc. (Roche Holding AG)
• Original Assignee: Osmetech Technology Inc. (Roche Holding AG)
• Inventors: Kayyem, Jon Faiz, O'Connor, Stephen D., Meade, Thomas J.
• Primary Examiner(s): Cook; Lisa V
• Application Number:
  US11/981,735
• Publication Number:
  US 20080237061A1
• Time in Patent Office: 924 Days
• Field of Search: None
• US Class Current: 435/7.1
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 2458/30 Electrochemically active labels
• CPC Class Codes: G01N 27/3277 being a redox reaction, e.g. detection by cyclic voltammetry voltammetry per se G01N27/42, G01N27/48
• CPC Class Codes: G01N 2800/04 Endocrine or metabolic disorders
• CPC Class Codes: G01N 33/5438 Electrodes
• CPC Class Codes: G01N 33/58 involving labelled substances G01N33/53 takes precedence
• CPC Class Codes: G01N 33/84 involving inorganic compounds or pH

Abstract

The present invention is directed to the detection of target analytes using electronic techniques, particularly AC techniques.

Claims

1. An apparatus for the detection of a non-nucleic acid target analyte in a test sample, comprising:

a) a test chamber comprising gold electrodes, each electrode comprises;

a covalently attached passivation species comprising a protein binding ligand;

wherein said protein binding ligand is covalently attached to said electrode via a spacer,

wherein said spacer is attached to said electrode with more than one sulfur moiety, and

wherein said test chamber further comprises at least one second measuring electrode,

b) a voltage source electrically connected to said test chamber; and

c) an electronic detector.

2. The apparatus according to claim 1, further comprising a processor coupled to said electrodes and configured to receive an output signal.

3. The apparatus according to claim 1, wherein said spacer is attached to said electrode using two sulfur moieties.

4. The apparatus according to claim 1, wherein said protein binding ligand comprises a protein.

5. The apparatus according to claim 4, wherein said protein is a peptide.

6. The apparatus according to claim 1, wherein said protein binding ligand comprises a substrate of an enzyme.

7. The apparatus according to claim 1, wherein said passivation species comprises an insulator.

8. The apparatus according to claim 7, wherein said insulator comprises a C1 to C12 alkyl group.

9. The apparatus according to claim 1, wherein said passivation species comprises a conductive oligomer.