Application using non-covalent bond between a cucurbituril derivative and a ligand

• US 7,718,128 B2
• Filed: 12/19/2008
• Issued: 05/18/2010
• Est. Priority Date: 10/20/2005
• Status: Active Grant

• Current Assignee: Postech Foundation
• Original Assignee: Postech Foundation
• Inventors: Kim, Jeeyeon, Hwang, Ilha, Kim, Kimoon, Baek, Kangkyun, Park, Kyeng Min, Selvapalam, Narayanan, Ko, Young Ho, Nagarajan, Erumaipatty R.
• Primary Examiner(s): Riley; Jezia
• Application Number:
  US12/339,944
• Publication Number:
  US 20090095677A1
• Time in Patent Office: 515 Days
• Field of Search: 435/5, 435/6, 435/7.1, 435/7.2, 422/61, 422/68.1, 424/278.1, 424/280, 424/486, 424/489
• US Class Current: 422/68.1
• CPC Class Codes: B01D 15/3804 Affinity chromatography
• CPC Class Codes: B01J 31/0249 Ureas (R2N-C(=O)-NR2)
• CPC Class Codes: B01J 31/0251 Guanidides (R2N-C(=NR)-NR2)
• CPC Class Codes: B01J 31/1658 immobilised by covalent linkages, i.e. pendant complexes with optional linking groups, e.g. on Wang or Merrifield resins
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 5/00 Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
• CPC Class Codes: C07D 487/22 in which the condensed system contains four or more hetero rings
• CPC Class Codes: C07K 1/22 Affinity chromatography or related techniques based upon selective absorption processes

Abstract

Provided are a kit including a first component that is a compound of formula (1) below bound to a first material and a second component that is a ligand bound to a second material, wherein each of the first and second materials is independently selected from the group consisting of a solid phase, a biomolecule, an antioxidant, a chemical therapeutic agent, an anti-histaminic agent, a cucurbituril dendrimer, a cyclodextrin derivative, a crown ether derivative, a calixarene derivative, a cyclophane derivative, a cyclic peptide derivative, a metallic ion, a chromophore, a fluorescent material, a phosphor, a radioactive material, and a catalyst; and the ligand can non-covalently bind to the compound of formula (1); a method of separating and purifying a material bound to a ligand using the compound of formula (1) bound to a solid phase; a method of separating and purifying the compound of formula (1) or a material bound to the compound using a ligand bound to a solid phase; a sensor chip including a compound of formula (1) bound to a first material and a ligand bound to a second material; and a solid-catalyst complex including the compound of formula (1) bound to a first material and a ligand bound to a second material.

Claims

1. A sensor chip comprising a compound of formula (1) below bound to a first material and a ligand bound to a second material, wherein one of the first and second materials is a solid phase, and the other is selected from the group consisting of an enzyme including histidine, cystein, or tryptophane, a substrate, a substrate analogue, a suppressor, a coenzyme, an antibody, an antigen, a virus, cell lectin, a polysaccharide, a glucoprotein, a cell surface receptor, a nucleic acid, a complementary base sequence, histone, a nucleic acid polymerase, a nucleic acid binding protein, ATP, ADP, a hormone, a vitamine, a receptor, a carrier protein, glutathione, a GST fusion protein, a metallic ion, a polyHIS fusion protein, a natural protein, and a combination thereof,

2. The sensor chip of claim 1, wherein the solid phase is a gold thin film, a silver thin film, or an ITO-coated glass.

3. A solid-catalyst complex comprising a compound of formula (1) below bound to a first material and a ligand bound to a second material, wherein one of the first and second materials is a solid phase, and the other is a catalyst,

4. The solid-catalyst complex of claim 3, wherein the solid phase is selected from the group consisting of a polymer, a resin, a magnetic material, a silicagel, a polymer- or gold-coated silicagel, a zirconium oxide, a monolithic polymer, a polymer-coated magnetic particle, a gold thin film, a silver thin film, glass, an ITO-coated glass, silicon, a metal electrode, a nanorod, a nanotube, a nanowire, curdlan gum, cellulose, a nylon film, sepharose, and sephadex.

5. The solid-catalyst complex of claim 4, wherein the solid phase is polystyrene resin or polymer-coated silicagel.

6. The solid-catalyst complex of claim 3, wherein the catalyst is a Grubbs catalyst, a radical initiator, or a combination thereof.

7. The solid-catalyst complex of claim 3, wherein the catalyst is selected from the group consisting of cellulase, hemicellulase, peroxidase, protease, amylase, xylanase, lipase, esterase, cutinase, pectinase, keratinase, reductase, oxidase, phenoloxidase, lipoxigenase, ligninase, pullulanase, arabinosidase, hyaluronidase, and a combination thereof.