DNA chip based genetic typing
First Claim
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1. A method for medium resolution typing of a target human leukocyte antigen (HLA) gene comprising:
- a) isolating a target leukocyte cell comprising a target HLA gene from a suitable sample and obtaining a preparation comprising a target HLA nucleotide sequence that is at least a part of said target HLA gene from said isolated target leukocyte cell and, optionally another nucleotide sequence not related to said target HLA gene;
b) providing a chip comprising a support suitable for use in nucleic acid hybridization having immobilized thereon;
a set of oligonucleotide probes complementary to said target HLA nucleotide sequence, each of said probes having 30 nucleotides or less, wherein said set of oligonucleotide probes comprises SEQ ID NOS;
1-214 or the complement of SEQ ID NOS;
1-214; and
at least one of each of the following oligonucleotide control probes;
a positive control probe, a negative control probe, a hybridization control probe and an immobilization control probe; and
c) hybridizing said preparation obtained in step a) to said chip provided in step b) and assessing hybridization between said target HLA nucleotide sequence and/or said another nucleotide sequence and said probe comprised on said chip to determine the type of said target HLA gene.
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Abstract
This invention relates generally to the field of nucleic acid analysis. In particular, the invention provides a method for typing a target gene, using, inter alia, a chip comprising a support suitable for use in nucleic acid hybridization having immobilized thereon an oligonucleotide probe complementary to said target nucleotide sequence and at least one of the following oligonucleotide control probes: a positive control probe, a negative control probe, a hybridization control probe and an immobilization control probe. Oligonucleotide probes or probes arrays for typing a HLA target gene are also provided.
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Citations
23 Claims
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1. A method for medium resolution typing of a target human leukocyte antigen (HLA) gene comprising:
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a) isolating a target leukocyte cell comprising a target HLA gene from a suitable sample and obtaining a preparation comprising a target HLA nucleotide sequence that is at least a part of said target HLA gene from said isolated target leukocyte cell and, optionally another nucleotide sequence not related to said target HLA gene; b) providing a chip comprising a support suitable for use in nucleic acid hybridization having immobilized thereon;
a set of oligonucleotide probes complementary to said target HLA nucleotide sequence, each of said probes having 30 nucleotides or less, wherein said set of oligonucleotide probes comprises SEQ ID NOS;
1-214 or the complement of SEQ ID NOS;
1-214; and
at least one of each of the following oligonucleotide control probes;
a positive control probe, a negative control probe, a hybridization control probe and an immobilization control probe; andc) hybridizing said preparation obtained in step a) to said chip provided in step b) and assessing hybridization between said target HLA nucleotide sequence and/or said another nucleotide sequence and said probe comprised on said chip to determine the type of said target HLA gene. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23)
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Specification