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Microarray analysis of RNA

  • US 7,718,365 B2
  • Filed: 07/09/2005
  • Issued: 05/18/2010
  • Est. Priority Date: 07/09/2005
  • Status: Active Grant
First Claim
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1. A method of analyzing RNAs comprising:

  • a) contacting a sample comprising RNAs and interfering sequences that are complementary to said RNAs with an analogous DNA set to provide a DNA/RNA duplex comprising an analogous DNA of said set and a complementary interfering sequence, wherein the analogous DNA set comprises at least one sequence comprising the same sequence of bases as an RNA of said sample, except that uracils in the sequence of said RNA are substituted with thymines, and wherein said sample is isolated from a biological source;

    b) contacting the DNA/RNA duplex with an enzyme having a DNA;

    RNA nuclease activity to provide a digested RNA sample;

    c) contacting the digested RNA sample with an array of probes that are complementary to the analogous DNAs of said analogous DNA set under conditions sufficient to provide for specific binding of said RNA to a probe of said array; and

    d) interrogating the array.

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