Methods and compositions for directed microwave chemistry

• US 7,718,445 B2
• Filed: 03/31/2008
• Issued: 05/18/2010
• Est. Priority Date: 10/03/2000
• Status: Expired due to Fees

• Current Assignee: Milestone S.R.L. (Embracer Group AB)
• Original Assignee: Mirari Biosciences, Inc.
• Inventors: Martin, Mark
• Primary Examiner(s): Shibuya; Mark L
• Assistant Examiner(s): Do; Pensee T
• Application Number:
  US12/059,427
• Publication Number:
  US 20080248489A1
• Time in Patent Office: 778 Days
• Field of Search: 436/518, 436/173, 436/806, 436/50, 436/51, 436/526, 436/149, 435/4, 435/6, 435/7.1, 435/287.1, 435/287.2
• US Class Current: 436/518
• CPC Class Codes: C12N 11/00 Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
• CPC Class Codes: C12N 13/00 Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
• CPC Class Codes: Y10S 436/806 Electrical property or magnetic property
• CPC Class Codes: Y10T 436/115831 Condition or time responsive
• CPC Class Codes: Y10T 436/116664 with automated titrator
• CPC Class Codes: Y10T 436/24 Nuclear magnetic resonance, electron spin resonance or other spin effects or mass spectrometry

Abstract

The present invention concerns a novel means by which specific chosen reactions can be accelerated through the use of a new type of artificial enzyme. The invention allows specific reactions to occur at an accelerated rate, even in the presence of other non-chosen molecules, which may be very similar in structure to the chosen reactant. The reactions may be stoichiometric or catalytic.

Claims

1. A method for detecting a chemical reaction of a reactant, said method comprising:

(a) contacting said reactant with a solid composite, said composite comprising a material susceptible to microwave heating and a specific binding molecule for the reactant, said contacting being conducted under conditions sufficient to allow said reactant to bind to said specific binding molecule of said composite via a biospecific interaction, wherein said specific binding molecule is thermally proximal to said solid material to thereby provide the reactant thermally proximal to the solid material upon binding;

(b) applying an electromagnetic field of a wavelength of between 1 cm and 100 m to said composite, resulting in microwave heating of said solid material, wherein said heated solid material heats said thermally proximal reactant, wherein said chemical reaction is accelerated by said heating of said reactant; and

(c) detecting a physical or chemical property associated with said chemical reaction.

2. The method of claim 1, wherein said step of contacting comprises mixing said composite into a solution containing said reactant.

3. The method of claim 1, wherein said chemical reaction is hydrolysis, homolytic cleavage, or a chemiluminescent reaction.

4. The method of claim 1, wherein said reactant is capable of producing a physical or chemical property associated with said chemical reaction.

5. The method of claim 4, wherein the physical or chemical property associated with said chemical reaction is color, luminescence, fluorescence or electrochemistry.

6. The method of claim 4, wherein the production of the physical or chemical property is accelerated by said heating of said reactant.

7. The method of claim 1, wherein said step of detecting further comprises detecting a change in the physical or chemical property associated with said chemical reaction.

8. The method of claim 1, wherein said step of detecting comprises the use of x-ray film, a photomultiplier tube (PMT) or a charge-couple device (COD) camera.

9. The method of claim 1, wherein said solid material is selected from the group consisting of carbon, charcoal, amorphous carbon, carbon black, clay, and nickel.

10. The method of claim 1, wherein said solid material is an oxide selected from the group consisting of copper oxide, chromium oxide, silicon oxide, niobate oxide and manganese oxide.

11. The method of claim 1, wherein said solid material is a titanate selected from the group consisting of barium titanate, and an inorganic titanate.

12. The method of claim 1, wherein said solid material is an oxide or non-oxide ceramic, a ferrite, a ferroelectric polymer, or an organic polymer.

13. The method of claim 1, wherein said solid material is selected from the group consisting of SiC, Si, Mg, FeSi, Cr₂O₃, Fe₃O₄, MnO₂, NiO.

14. The method of claim 1, wherein said solid material is a mixture of a conductive material and an insulator.

15. The method of claim 1, wherein said specific binding molecule is selected from the group consisting of a molecularly imprinted polymer, a zeolite, an antibody, a modified antibody, an enzyme, a modified enzyme, a cavitand, a chiral ligand, a low molecular weight organic synthetic receptor, single stranded nucleic acid, and double stranded nucleic acid.

16. The method of claim 1, wherein said reactant is selected from the group consisting of an antigen, receptor ligand, enzyme inhibitor, enzyme substrate, enzyme product, and oligonucleotide.

17. The method of claim 1, wherein said reactant is a covalent conjugate of a small molecule and a molecule selected from the group consisting of a molecularly imprinted polymer, a zeolite, an antibody, a modified antibody, an enzyme, a modified enzyme, a cavitand, a chiral ligand, a low molecular weight organic synthetic receptor, single stranded nucleic acid, and double stranded nucleic acid.

18. The method of claim 1, wherein said specific binding molecule has at least one binding site for said reactant, said binding site facilitating more than one reaction turnover.

19. The method of claim 1, wherein said specific binding molecule has at least one binding site for said reactant, said binding site facilitating one reaction turnover.

20. The method of claim 1, wherein said electromagnetic field is applied at a frequency of from about 0.9 to about 25 GHz.

21. The method of claim 20, wherein said electromagnetic field is applied at a frequency of from about 0.9 to about 6 GHz.

22. The method of claim 21, wherein said electromagnetic field is applied at a frequency of from about 0.9 to about 2.5 GHz.

23. The method of claim 20, wherein said electromagnetic field is applied at a frequency selected from the group consisting of 0.915, 2.45, 5.85 and 22.125 GHz.

24. The method of claim 1, in which said composite is in the form of a planar substrate.

25. The method of claim 1, wherein said solid material susceptible to microwave heating comprises a dielectric material, and wherein said application of said electromagnetic field additionally results in dielectric heating.

26. The method of claim 1, wherein said solid material is more susceptible to microwave heating than water.

27. A method for detecting a chemical reaction of a reactant, said method comprising:

(a) contacting a reactant-containing solution with a solid composite, said composite comprising a specific binding molecule for the reactant and a material susceptible to microwave heating, said contacting being under conditions sufficient to allow said reactant to bind to said specific binding molecule of said composite via a biospecific interaction, thereby forming a composite-reactant complex, wherein said composite-reactant complex is thermally proximal to said solid material;

(b) separating said composite-reactant complex from the solution;

(c) applying an electromagnetic field of a wavelength of between 1 cm and 100 m to said composite of said composite-reactant complex, resulting in microwave heating of the solid material of said complex,—

whereby said heated solid material heats said reactant of said thermally proximal composite—

reactant complex, wherein said chemical reaction is accelerated by said heating of said reactant of said composite-reactant complex; and

(d) detecting a physical or chemical property associated with said chemical reaction.

28. The method of claim 27, wherein said chemical reaction is hydrolysis, homolytic cleavage, or a chemiluminescent reaction.

29. The method of claim 27, wherein said reactant is capable of producing a physical or chemical property associated with said chemical reaction.

30. The method of claim 27, wherein the physical or chemical property associated with said chemical reaction is color, luminescence, fluorescence or electrochemistry.

31. The method of claim 27, wherein the production of the physical or chemical property is accelerated by said heating of said reactant.

32. The method of claim 27, wherein said step of detecting further comprises detecting a change in the physical or chemical property associated with said chemical reaction.

33. The method of claim 27, wherein said step of detecting comprises the use of x-ray film, a photomultiplier tube (PMT) or a charge-couple device (COD) camera.

34. The method of claim 27, wherein said solid material is selected from the group consisting of carbon, charcoal, amorphous carbon, carbon black, clay, and nickel.

35. The method of claim 27, wherein said solid material is an oxide selected from the group consisting of copper oxide, chromium oxide, silicon oxide, niobate oxide and manganese oxide.

36. The method of claim 27, wherein said solid material is a titanate selected from the group consisting of barium titanate, and an inorganic titanate.

37. The method of claim 27, wherein said solid material is an oxide or non-oxide ceramic, a ferrite, a ferroelectric polymer, or an organic polymer.

38. The method of claim 27, wherein said solid material is selected from the group consisting of SiC, Si, Mg, FeSi, Cr₂O₃, Fe₃O₄, MnO₂, NiO.

39. The method of claim 27, wherein said solid material is a mixture of a conductive material and an insulator.

40. The method of claim 27, wherein said specific binding molecule is selected from the group consisting of a molecularly imprinted polymer, a zeolite, an antibody, a modified antibody, an enzyme, a modified enzyme, a cavitand, a chiral ligand, a low molecular weight organic synthetic receptor, single stranded nucleic acid, and double stranded nucleic acid.

41. The method of claim 27, wherein said reactant is selected from the group consisting of an antigen, receptor ligand, enzyme inhibitor, enzyme substrate, enzyme product, and oligonucleotide.

42. The method of claim 27, wherein said reactant is a covalent conjugate of a small molecule and a molecule selected from the group consisting of a molecularly imprinted polymer, a zeolite, an antibody, a modified antibody, an enzyme, a modified enzyme, a cavitand, a chiral ligand, a low molecular weight organic synthetic receptor, single stranded nucleic acid, and double stranded nucleic acid.

43. The method of claim 27, wherein said specific binding molecule has at least one binding site for said reactant, said binding site facilitating more than one reaction turnover.

44. The method of claim 27, wherein said specific binding molecule has at least one binding site for said reactant, said binding site facilitating one reaction turnover.

45. The method of claim 27, wherein said electromagnetic field is applied at a frequency of from about 0.9 to about 25 GHz.

46. The method of claim 45, wherein said electromagnetic field is applied at a frequency of from about 0.9 to about 6 GHz.

47. The method of claim 46, wherein said electromagnetic field is applied at a frequency of from about 0.9 to about 2.5 GHz.

48. The method of claim 45, wherein said electromagnetic field is applied at a frequency selected from the group consisting of 0.915, 2.45, 5.85 and 22.125 GHz.

49. The method of claim 27, in which said composite is in the form of a planar substrate.

50. The method of claim 27, wherein said solid material susceptible to microwave heating comprises a dielectric material, and wherein said application of said electromagnetic field additionally results in dielectric heating.

51. The method of claim 27, wherein said solid material is more susceptible to microwave heating than water.