Bioagent detection device

• US 7,722,809 B2
• Filed: 06/03/2005
• Issued: 05/25/2010
• Est. Priority Date: 06/04/2004
• Status: Active Grant

• Current Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Original Assignee: Wisconsin Alumni Research Foundation (University of Wisconsin System)
• Inventors: Beebe, David J., Jiang, Hongrui
• Primary Examiner(s): JARRETT, LORE RAMILLANO
• Application Number:
  US11/144,723
• Publication Number:
  US 20060078462A1
• Time in Patent Office: 1,817 Days
• Field of Search: 422/58, 422/63, 435/7.1, 73/530.1, 251/12, 137/14, 137/3, 436/169
• US Class Current: 422/504
• CPC Class Codes: B01L 2300/0645 Electrodes
• CPC Class Codes: B01L 2400/0677 phase change valves; Meltable, freezing, dissolvable plugs; Destructible barriers
• CPC Class Codes: B01L 3/502738 characterised by integrated valves microvalves F16K99/0001
• CPC Class Codes: G01N 33/48707 by electrical means G01N33/49, G01N33/493 take precedence
• CPC Class Codes: Y10T 137/0329 Mixing of plural fluids of diverse characteristics or conditions
• CPC Class Codes: Y10T 137/0396 Involving pressure control

Abstract

A detection device is provided for detecting the presence of an agent in a fluid. The device includes a body defining first and second chambers. It is intended for the first chamber accommodating the flow of fluid therein. A first valve is disposed in the body between in the first and second chambers. The first valve opens in response to the presence of the agent in the fluid. A first detection structure is disposed in the second chamber in order to generate a predetermined signal in response to exposure to the fluid.

Claims

1. A detection device for detecting the presence of a bioagent in a fluid, comprising:

a body defining a first chamber for accommodating fluid therein and a second chamber;

a detection structure disposed in the second chamber of the body, the detection structure generating a predetermined signal in response to exposure to the fluid, wherein the detection structure includes a microcapacitor having first and second terminals for connecting the detection structure to a signal detection circuit; and

a valve interconnecting the first and second chambers, the valve dissolving and becoming a solute in the fluid in response to the presence of the bioagent in the fluid in the second chamber, wherein the valve is a membrane material comprising peptide sequences to serve as recoginition elements for the bioagent to be detected.

2. The device of claim 1 wherein the microcapacitor includes first and second spaced electrodes.

3. The device of claim 1 wherein fluid flows into the second chamber with the valve dissolved and wherein the microcapacitor has a first capacitance in the absence of fluid in the second chamber and a second capacitance with the fluid in the second chamber.

4. The device of claim 1 wherein the body defines a channel having an input communicating with the first chamber and an output communicating with the second chamber and wherein the valve includes a dissolvable member in the first chamber overlapping the input to the channel.

5. The device of claim 1 wherein the body defines a third chamber and wherein the device further comprises:

a second detection structure disposed in the third chamber of the body, the detection structure generating a predetermined signal in response to exposure to the fluid; and

a second valve interconnecting the second and third chambers, the second valve opening in response to the presence of a second bioagent in the fluid in the second chamber.

6. The device of claim 1 wherein the body defines a third chamber and wherein the device further comprises:

a second detection structure disposed in the third chamber of the body, the detection structure generating a predetermined signal in response to exposure to the fluid; and

a second valve interconnecting the second and third chambers, the second valve opening in response to the presence of the bioagent in the fluid in the second chamber.

7. The device of claim 6 wherein the first valve opens a first predetermined time period after exposure to the bioagent and the second valve opens a second predetermined time period after exposure to the bioagent and wherein the first predetermined time period is less than the second predetermined time period.

8. The device of claim 6 wherein the first and second detection structures are connected in parallel.

9. A detection device for detecting the presence of a bioagent in a fluid, comprising:

a body defining first and second chambers, the first chamber accommodating the flow of fluid therein;

a first valve disposed in the body, the first valve;

having a first closed configuration wherein the first and second chambers are isolated and a second open configuration wherein the first and second chambers communicate; and

including a polymeric material isolating the first and second chambers with the first valve in the closed configuration, the polymeric material dissolving and becoming a solute in the fluid in response to exposure to the bioagent, wherein the polymeric material comprises peptide sequences to serve as recognition elements for the bioagent to be detected;

a first detection structure disposed in the second chamber, the first detection structure generating a predetermined signal in response exposure to the fluid, wherein the first detection structure includes a microcapacitor having first and second terminals for connecting the first detection structure to a signal detection circuit.

10. The device of claim 9 wherein the body further defines a channel extending between the first and second chamber, the channel having an input communicating with the first chamber and an output communication with the second chamber and wherein the first valve overlaps the input to the channel.

11. The device of claim 9 wherein the second chamber accommodates the flow of fluid therein with the valve in the open configuration and wherein the microcapacitor has a first capacitance in the absence of fluid in the second chamber and a second capacitance with the fluid in the second chamber.

12. The device of claim 9 wherein the body defines a third chamber and wherein the device further comprises:

a second valve disposed in the body, the valve having a first closed configuration wherein the first and third chambers are isolated and a second open configuration wherein the first and third chambers communicate; and

a second detection structure disposed in the second chamber, the second detection structure generating a predetermined signal in response exposure to the fluid.

13. The device of claim 12 wherein the second valve is formed from a second polymeric material that isolates the first and third chambers, the second polymeric material dissolving in response to exposure to a second bioagent.

14. The device of claim 12 wherein the first valve opens a first predetermined time period after exposure to the bioagent and the second valve opens a second predetermined time period after exposure to the bioagent and wherein the first predetermined time period is less than the second predetermined time period.

15. The device of claim 12 wherein the first and second detection structures are connected in parallel.

16. A method of detecting the presence of a bioagent in a fluid, comprising:

passing the fluid into a body defining first and second chambers, the first and second chambers being isolated from each other by a first valve formed from a polymeric material, wherein the polymeric material comprises peptide sequences to serve as recognition elements for the bioagent to be detected;

dissolving the polymeric material into a solute in the fluid in response to exposure to the bioagent in the fluid so as to allow fluid to flow into the second chamber;

generating a signal in response to the presence of fluid in the second chamber,

wherein the step of generating a signal includes the additional steps of;

providing a microcapacitor having an initial capacitance in the second chamber;

varying the capacitance of the microcapacitor in response to the presence of fluid in the second chamber; and

detecting the change in capacitance.

17. The method of claim 16 comprising the additional steps of:

providing a third chamber in the body;

opening a second valve in response to a second bioagent in the fluid so as to allow fluid to flow into the third chamber; and

generating a second signal in response to the presence of fluid in the third chamber.

18. The method of claim 16 comprising the additional steps of:

providing a third chamber in the body;

opening a second valve in response to the bioagent in the fluid so as to allow fluid to flow into the third chamber; and

varying the signal in response to the presence of fluid in the third chamber.

19. The method of claim 18 wherein the polymeric material of the first valve is a first polymeric material that isolates the first and second chambers, the first polymeric material dissolving in a first predetermined time period in response to exposure to the bioagent and wherein the second valve is formed from a second polymeric material that isolates the first and third chambers, the second polymeric material dissolving in a second predetermined time period in response to exposure to the bioagent.

20. The method of claim 18 wherein the first and second valves open sequentially in response to exposure to the bioagent in the fluid.