Synthesis of arrays of oligonucleotides and other chain molecules
First Claim
1. Apparatus for synthesis of arrays of chain molecules comprising:
- (a) a substrate with an active surface on which the arrays may be formed;
(b) a light emitting object array configured to emit a pattern of light and dark areas; and
(c) projection optics configured to receive the light emitted from the light emitting object array and to image the pattern of light and dark areas onto the active surface of the substrate, the projection optics comprising a first, concave mirror configured to receive the light from the object array and to reflect that light at an angle off the axis of the light received from the object array, a second, convex mirror configured to receive the light reflected from the first mirror and to reflect the light off the axis of the light received from the first mirror, a third, concave mirror configured to receive the light reflected from the second mirror and to reflect the light off the axis of the light received from the second mirror, and a fourth, convex mirror configured to receive the light from the third mirror and to reflect the light off the axis of the light received from the third mirror and to image the light onto the active surface, the first, second, third and fourth mirrors arranged to form a magnified image of the pattern of light and dark areas at the active surface.
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Accused Products
Abstract
Synthesis of arrays of chain molecules, such as oligonucleotides, in large quantities can be carried out utilizing projection onto an active substrate of a magnified image of a light emitting object array having selectable regions of light and dark areas forming a pattern. Projection optics formed entirely of mirrors are used to receive the light emitted from the object array and image the pattern of the array onto the active surface of the substrate. The mirrors in the projection optics include a first, concave mirror, a second, convex mirror, a third, concave mirror, and a fourth, convex mirror, each receiving the beam of light in turn, with the light reflected from the fourth mirror being imaged onto the active surface of the substrate with an image area greater than that of the original light emitting array.
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Citations
22 Claims
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1. Apparatus for synthesis of arrays of chain molecules comprising:
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(a) a substrate with an active surface on which the arrays may be formed; (b) a light emitting object array configured to emit a pattern of light and dark areas; and (c) projection optics configured to receive the light emitted from the light emitting object array and to image the pattern of light and dark areas onto the active surface of the substrate, the projection optics comprising a first, concave mirror configured to receive the light from the object array and to reflect that light at an angle off the axis of the light received from the object array, a second, convex mirror configured to receive the light reflected from the first mirror and to reflect the light off the axis of the light received from the first mirror, a third, concave mirror configured to receive the light reflected from the second mirror and to reflect the light off the axis of the light received from the second mirror, and a fourth, convex mirror configured to receive the light from the third mirror and to reflect the light off the axis of the light received from the third mirror and to image the light onto the active surface, the first, second, third and fourth mirrors arranged to form a magnified image of the pattern of light and dark areas at the active surface. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12)
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13. Apparatus for synthesis of arrays of chain molecules comprising:
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(a) a substrate with an active surface on which the arrays may be formed; (b) a flow cell enclosing the active surface of the substrate and having ports for applying DNA synthesis reagents into the flow cell which can be flowed over the active surface of the substrate; (c) a light emitting object array comprising a light source and a micromirror device configured to receive a light beam from the light source and formed of an array of electronically addressable micromirrors, each of which can be selectively tilted between one of at least two separate positions, wherein in one of the positions of each micromirror the light from the source incident upon the micromirror is deflected away from an optical axis and in a second of the at least two positions of the micromirror the light is reflected along the optical axis; and (d) projection optics configured to receive the light emitted from the light emitting object array and to image the pattern of light and dark areas onto the active surface of the substrate, the projection optics comprising a first, concave mirror configured to receive the light from the object array and to reflect that light at an angle off the axis of the light received from the object array, a second, convex mirror configured to receive the light reflected from the first mirror and to reflect the light off the axis of the light received from the first mirror, a third, concave mirror configured to receive the light reflected from the second mirror and to reflect the light off the axis of the light received from the second mirror, and a fourth, convex mirror configured to receive the light from the third mirror and to reflect the light off the axis of the light received from the third mirror and to image the light onto the active surface, the first, second, third and fourth mirrors arranged to form a magnified image of the pattern of light and dark areas at the active surface. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22)
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Specification