Chloramphenicol acetyl transferase (CAT)-defective somatostatin fusion protein and uses thereof

• US 7,722,881 B2
• Filed: 08/26/2008
• Issued: 05/25/2010
• Est. Priority Date: 06/25/2008
• Status: Active Grant

• Current Assignee: Braasch Biotech LLC
• Original Assignee: Braasch Biotech LLC
• Inventors: Larrick, James, Mendelsohn, Andrew R., Haffer, Keith N.
• Primary Examiner(s): Graser; Jennifer E
• Application Number:
  US12/198,579
• Publication Number:
  US 20090324629A1
• Time in Patent Office: 637 Days
• Field of Search: None
• US Class Current: 424/198.1
• CPC Class Codes: A23K 50/60 for weanlings
• CPC Class Codes: A61K 2039/552 Veterinary vaccine
• CPC Class Codes: A61K 2039/55555 Liposomes; Vesicles, e.g. nanoparticles; Spheres, e.g. nanospheres; Polymers
• CPC Class Codes: A61K 2039/55566 Emulsions, e.g. Freund's adjuvant, MF59
• CPC Class Codes: A61K 38/31 Somatostatins
• CPC Class Codes: A61K 39/0005 Vertebrate antigens from snakes A61K39/38
• CPC Class Codes: A61P 37/00 Drugs for immunological or allergic disorders
• CPC Class Codes: A61P 37/04 Immunostimulants
• CPC Class Codes: C07K 14/655 Somatostatins
• CPC Class Codes: C07K 2319/00 Fusion polypeptide
• CPC Class Codes: C12N 9/1033 Chloramphenicol O-acetyltransferase (2.3.1.28)
• CPC Class Codes: C12Y 203/01028 Chloramphenicol O-acetyltransferase (2.3.1.28)

Abstract

Chimeric somatostatin-based polypeptides, polynucleotides used to encode the polypeptides, the methods for isolating and producing the polypeptides and the uses thereof are provided. In addition, low cost adjuvants for enhanced immunogenic response are provided. Vaccinations that include both chimeric somatostatin-based polypeptides and novel adjuvants are included, useful in facilitating farm animal productivity.

Claims

1. A chimeric polypeptide having the immunogenicity of somatostatin, comprising:

an amino acid sequence of somatostatin-14 represented by SEQ ID NO;

1 linked to a substantially inactive and truncated chloramphenicol acetyl transferase polypeptide having a sequence selected from the group consisting of SEQ ID NO;

4, SEQ ID NO;

8 and SEQ ID NO;

9,

wherein the somatostatin-14 is linked to the inactive chloramphenicol acetyl transferase by a spacer sequence selected from SEQ ID NO;

11 or SEQ ID NO;

12.

2. The chimeric polypeptide of claim 1 having an amino acid sequence with at least 99% sequence identity with the amino acid sequence of SEQ ID NO:

14.

3. The chimeric polypeptide of claim 1 having an amino acid sequence of SEQ ID NO:

14.

4. An immunogenic composition comprising the chimeric polypeptide of claim 1 together with a pharmaceutically suitable adjuvant in an amount effective to elicit an immune response.

5. A chimeric polypeptide comprising:

an amino acid sequence selected from the group consisting of SEQ ID NO;

4, 8, and 9; and

an amino acid sequence of SEQ ID NO;

1;

wherein a spacer selected from the group consisting of SEQ ID NO;

11 and SEQ TD NO;

12 links the amino acid sequences.