Optical sensor array system and method for parallel processing of chemical and biochemical information

• US 7,723,120 B2
• Filed: 10/26/2005
• Issued: 05/25/2010
• Est. Priority Date: 10/26/2005
• Status: Active Grant

• Current Assignee: BL Technologies Inc. (Ashland Global Holdings, Inc.)
• Original Assignee: General Electric Company
• Inventors: Shrikhande, Prashant V., Leach, Andrew M., Cecconie, Theodore J., Morris, William G., Potyrailo, Radislav A., Xiao, Caibin, Yu, LiJie, Boyette, Scott M.
• Primary Examiner(s): JARRETT, LORE RAMILLANO
• Application Number:
  US11/259,643
• Publication Number:
  US 20070092407A1
• Time in Patent Office: 1,672 Days
• Field of Search: 422/82.05, 382/273, 435/6, 436/164, 436/518, 137/375
• US Class Current: 436/164
• CPC Class Codes: B01L 2200/0621 Control of the sequence of chambers filled or emptied
• CPC Class Codes: B01L 2300/0803 Disc shape
• CPC Class Codes: B01L 2300/0816 Cards, e.g. flat sample carriers usually with flow in two horizontal directions
• CPC Class Codes: B01L 2300/0864 comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/088 by specific surface properties
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043
• CPC Class Codes: B01L 3/502746 characterised by the means for controlling flow resistance, e.g. flow controllers, baffles B01L3/502738 takes precedence
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/78 producing a change of colour
• CPC Class Codes: G01N 21/80 Indicating pH value
• CPC Class Codes: G01N 31/22 using chemical indicators G01N31/02 takes precedence
• CPC Class Codes: Y10T 137/7036 Jacketed

Abstract

Total analysis systems and methods for simultaneously monitoring a suite of biological and/or chemical species in water and/or other process systems are disclosed. The system provides a sample-volume controlled sensor array comprising a fluid delivery device and a plurality of optical sensor elements for determining the presence and total concentrations of multiple analytes in the process system simultaneously. Image identification algorithms are provided for identifying the analytes based on image intensity, color pattern, positional arrangement, and the like. The methods incorporate multivariate optimization algorithms to analyze multiple sensor responses. This produces analytical results that are typically difficult to obtain without full system or variable compensation. The improved array response may then be utilized to measure, monitor, and control the concentration of analytes in the chemical or biological sample or water system.

Claims

1. A method for simultaneously measuring a plurality of chemical or biological analytes in a sample liquid, said method comprising the steps of:

providing an optical sensor array comprising a plurality of solid-film sensor elements for responding to at least one of said plurality of analytes;

attaching a sample distribution device to the sensor array, the sample distribution device forming a plurality of fluidic channels leading from a sample entry port to a plurality of reservoirs aligned with said sensor elements, wherein a cover layer of the sample distribution device has a hydrophobic outer surface, a hydrophilic lower surface, and a plurality of vent holes aligned with said reservoirs;

introducing said sample liquid into said sample distribution device through the sample entry port;

driving said sample liquid along said channels by the hydrophilic surface of the cover layer and into said reservoirs;

creating a chemical reaction between said at least one analyte and at least one reagent in said sensor elements;

filling each reservoir by accumulating the sample liquid over the top hydrophilic surface above the reservoir to form a pendant droplet,

allowing gravitational force to enable the droplet to reach a bottom wall of the reservoir, and then using capillary forces originating from the side walls of the reservoir to drive the sample liquid to fill the entire reservoir while air escapes through the vent hole as the reservoir fills with sample liquid, wherein the hydrophobic outer surface prevents sample liquid from flowing through the vent holes such that the vent holes serve as capillary barriers by impeding the sample liquid from flowing through the vent holes to reach the hydrophobic outer surface of the cover layer, thereby allowing a hydrostatic balance to be reached such that a fixed volume of sample liquid is delivered to each reservoir and sensor element without requiring an external force other than gravity to move the sample through the sample distribution device;

generating an optical response from said sensor elements;

recording said optical response; and

utilizing said optical response to measure, monitor, and control concentration of said analytes in said sample.

2. The method of claim 1, further comprising the step of directing said sample through capillary barriers between said entry port and said reservoirs.

3. The method of claim 2, further comprising the step of optimizing channel and reservoir geometric parameters to control and meter flow of said sample.

4. The method of claim 1, wherein said reservoirs are positioned on top of said sensor elements.

5. The method of claim 1, wherein said channels and reservoirs are configured in a branched-type structure.

6. The method of claim 1, wherein said channels and reservoirs are configured in a series or parallel-series configuration.

7. The method of claim 1, further comprising the step of providing a waste reservoir and a delay channel.

8. The method of claim 1, wherein said solid-film sensor elements are deposited onto a substrate, and said reservoirs are positioned on top of said solid-film sensor elements.

9. The method of claim 1, wherein said recording step comprises use of a digital or analog imaging device.

10. The method of claim 1, further comprising the step of processing said optical response by using Fourier transform, wavelet transform, Kalman filtering, Savitsky-Golay smoothing, running mean, median, polynomial methods, or combinations thereof.

11. The method of claim 1, further comprising reducing interference from said sensor elements by applying multivariate analysis to said plurality of responses from each said sensor element, wherein said reducing step comprises using multiple sensor chemistries for each said analyte, and deconvoluting interference responses from said multiple sensor chemistries using chemometric analysis, response surface analysis, least square analysis or combinations thereof.

12. The method of claim 1, wherein said array response is dynamic or steady state.

13. The method of claim 1, wherein said sensor elements are a combination of reversible and irreversible sensor elements.

14. The method of claim 1, further comprising the step of utilizing a response of one analyte responsive sensor element to enhance analysis of the other analyte-responsive sensor elements.

15. The method of claim 1, further comprising the step of providing two or more sensor elements responsive to different analytes to determine concentration of common interferants.

16. The method of claim 1, wherein said optical response is obtained by using reflected light analysis, colorimetric analysis, scattered light analysis transmitted light analysis, fluorescence analysis, chemoluminescence analysis, photoluminescence analysis, polarimetric analysis, or combinations thereof.

17. The method of claim 16, wherein said optical response is obtained in the spectral range from about 190 nm to about 10,000 nm.

18. The method of claim 16, wherein said optical response is obtained in the spectral range from about 200 nm to about 2,500 nm.

19. The method of claim 16, wherein said optical response is obtained in the spectral range from about 200 nm to about 1,500 nm.

20. The method of claim 1, further comprising the step of repeating steps at predetermined time intervals before, during, and after exposure to said sample to obtain a temporal response, and utilizing said temporal response to determine rates of response for said sensor array, and concentrations of analytes in said sample.

21. The method of claim 20, further comprising the step of utilizing said temporal response to identify system variables that are reflected in temporal changes in noise or interference information obtained from at least two-sensor array responses to improve control of said concentration of analytes in said substance.

22. The method of claim 21, wherein said temporal response is used to identify and eliminate outlier responses that do not reflect real system changes.

23. The method of claim 1, further comprising the step of providing an internal optical reference material to calibrate said array response of said sensor elements.

24. The method of claim 23, wherein said reference material comprises an optical response material comprising dyes, paints, and/or pigments.

25. The method of claim 23, wherein said reference material comprises an optical response material comprising inorganic, organic, and/or polymeric photonic crystals.

26. The method of claim 23, wherein said reference material is incorporated into said sensor elements or is positioned in proximity to said sensor elements.

27. The method of claim 1 further comprising depositing the plurality of sensor elements on a base plate and attaching the sample distribution device to the base plate, wherein the sample distribution device comprises the cover layer and a fluidic distribution layer, and wherein the fluidic distribution layer has a plurality of grooves and cutouts that form the channels and reservoirs.

28. The method of claim 27 further comprising providing a double-sided adhesive film with through holes cut to match said reservoirs in said fluidic distribution layer and binding said sensor film carrying base plate with said fluidic distribution layer using said adhesive film to create the plurality of reservoirs, each containing one of said sensor films, and channels connecting said reservoirs to said sample entry port.