Western blot by incorporating an affinity purification zone
First Claim
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1. A method for detecting one or more component of interest in a microfluidic system, the method comprising the steps of:
- (i) flowing a mixture of components, which mixture of components comprises the one or more component of interest, through a binding channel region, which binding channel region comprises a component-binding moiety and which is upstream from a separation channel region, thereby binding at least a portion of the one or more component of interest to the component-binding moiety;
(ii) flowing a remainder of the mixture of components through the separation channel region, resulting in one or more separated components, and detecting the one or more separated components;
(iii) releasing the one or more component of interest from the component-binding moiety, resulting in one or more released component of interest; and
,(iv) flowing the one or more released component of interest through the separation channel region and detecting the one or more released component of interest.
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Abstract
An upstream affinity purification region is used to bind one or more component of interest in a mixture of components prior to separating the mixture of components. Detection of the separated components and a released component of interest provide identification of the component of interest. In addition, post separation dilution is optionally used to improve detection of the mixture of components and the released component of interest. Microfluidic devices and systems suitable for performing such analyses are also provided.
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Citations
40 Claims
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1. A method for detecting one or more component of interest in a microfluidic system, the method comprising the steps of:
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(i) flowing a mixture of components, which mixture of components comprises the one or more component of interest, through a binding channel region, which binding channel region comprises a component-binding moiety and which is upstream from a separation channel region, thereby binding at least a portion of the one or more component of interest to the component-binding moiety; (ii) flowing a remainder of the mixture of components through the separation channel region, resulting in one or more separated components, and detecting the one or more separated components; (iii) releasing the one or more component of interest from the component-binding moiety, resulting in one or more released component of interest; and
,(iv) flowing the one or more released component of interest through the separation channel region and detecting the one or more released component of interest. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40)
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Specification