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Western blot by incorporating an affinity purification zone

  • US 7,723,123 B1
  • Filed: 05/31/2002
  • Issued: 05/25/2010
  • Est. Priority Date: 06/05/2001
  • Status: Active Grant
First Claim
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1. A method for detecting one or more component of interest in a microfluidic system, the method comprising the steps of:

  • (i) flowing a mixture of components, which mixture of components comprises the one or more component of interest, through a binding channel region, which binding channel region comprises a component-binding moiety and which is upstream from a separation channel region, thereby binding at least a portion of the one or more component of interest to the component-binding moiety;

    (ii) flowing a remainder of the mixture of components through the separation channel region, resulting in one or more separated components, and detecting the one or more separated components;

    (iii) releasing the one or more component of interest from the component-binding moiety, resulting in one or more released component of interest; and

    ,(iv) flowing the one or more released component of interest through the separation channel region and detecting the one or more released component of interest.

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