Highly sensitive proteomic analysis methods, and kits and systems for practicing the same
First Claim
1. A method of determining whether a sample includes at least one analyte of interest, said method comprising:
- (a) contacting said sample with an array of a plurality of distinct binding agents displayed on a surface of a solid support, wherein said sample is contacted with said array in the presence of a metal ion chelating polysaccharide and each of said binding agents at least comprises a specific epitope binding domain of an antibody;
(b) detecting the presence of any resultant binding complexes on said surface to obtain analyte binding data; and
(c) employing said analyte binding data to determine whether said sample includes said at least one analyte of interest;
wherein said method provides a sensitivity of at least 10 pg/ml of analyte of interest when said analyte is directly fluorescently labeled.
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Accused Products
Abstract
Methods of determining whether a sample includes one or more analytes, particularly proteinaceous analytes, of interest are provided. In the subject methods, an array of binding agents, where each binding agent includes an epitope binding domain of an antibody, is contacted with the sample. In many embodiments, contact occurs in the presence of a metal ion chelating polysaccharide, e.g., a pectin. Following contact, the presence of binding complexes on the array surface are detected and the resultant data is employed to determine whether the sample includes the one or more analytes of interest. Also provided are kits, systems and other compositions of matter for practicing the subject methods. The subject methods and compositions find use in a variety of applications, including proteomic applications such as protein expression analysis, e.g., differential protein expression profiling.
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Citations
18 Claims
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1. A method of determining whether a sample includes at least one analyte of interest, said method comprising:
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(a) contacting said sample with an array of a plurality of distinct binding agents displayed on a surface of a solid support, wherein said sample is contacted with said array in the presence of a metal ion chelating polysaccharide and each of said binding agents at least comprises a specific epitope binding domain of an antibody; (b) detecting the presence of any resultant binding complexes on said surface to obtain analyte binding data; and (c) employing said analyte binding data to determine whether said sample includes said at least one analyte of interest; wherein said method provides a sensitivity of at least 10 pg/ml of analyte of interest when said analyte is directly fluorescently labeled. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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Specification